ZFIN ID: ZDB-LAB-080603-1
Leung Lab
PI/Director: Leung, Yuk Fai
Contact Person: Leung, Yuk Fai
Email: yfleung@purdue.edu
URL: http://www.bio.purdue.edu/lab/leung/
Address: Department of Biological Sciences Purdue University LILY 2-136, 915 W. State Street West Lafayette, IN 47907
Country: United States
Phone: 765-496-3153
Fax: 765-494-0876
Line Designation: None assigned


Drug discovery for retinal degeneration

Retinal degeneration is a group of inherited eye diseases including retinitis pigmentosa and age-related macular degeneration that impair our vision. Although much has been learned about the molecular basis of these diseases, they are still incurable. To expedite discovery of new drugs for these diseases, our group at Purdue University studies zebrafish retinal-degeneration models.

The research interests of our lab are currently focused in two directions:

1. Rapid drug discovey for retinal degeneration

2. Gene network for disease and drug action

Please see our laboratory website for the latest development:

Ganzen, Logan Post-Doc Wang, Beichen (BC) Graduate Student Zhu, Xiaoguang Research Staff

Ganzen, L., Ko, M.J., Zhang, M., Xie, R., Chen, Y., Zhang, L., James, R., Mumm, J., van Rijn, R.M., Zhong, W., Pang, C.P., Zhang, M., Tsujikawa, M., Leung, Y.F. (2021) Drug screening with zebrafish visual behavior identifies carvedilol as a potential treatment for an autosomal dominant form of retinitis pigmentosa. Scientific Reports. 11:11432
Ko, M.J., Ganzen, L.C., Coskun, E., Mukadam, A.A., Leung, Y.F., van Rijn, R.M. (2019) A critical evaluation of TRPA1-mediated locomotor behavior in zebrafish as a screening tool for novel anti-nociceptive drug discovery. Scientific Reports. 9:2430
Xie, R., Zhang, M., Venkatraman, P., Zhang, X., Zhang, G., Carmer, R., Kantola, S.A., Pang, C.P., Ma, P., Zhang, M., Zhong, W., Leung, Y.F. (2019) Normalization of large-scale behavioural data collected from zebrafish. PLoS One. 14:e0212234
Weaver, C.J., Terzi, A., Roeder, H., Gurol, T., Deng, Q., Fai Leung, Y., Suter, D.M. (2018) nox2/cybb deficiency affects zebrafish retinotectal connectivity. The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(26):5854-5871
Huang, X.F., Xiang, L., Cheng, W., Cheng, F.F., He, K.W., Zhang, B.W., Zheng, S.S., Han, R.Y., Zheng, Y.H., Xu, X.T., Yu, H.Y., Zhuang, W., Leung, Y.F., Jin, Z.B. (2018) Mutation of IPO13 causes recessive ocular coloboma, microphthalmia, and cataract. Experimental & molecular medicine. 50:53
Liu, Y., Ma, P., Cassidy, P.A., Carmer, R., Zhang, G., Venkatraman, P., Brown, S.A., Pang, C.P., Zhong, W., Zhang, M., Leung, Y.F. (2017) Statistical Analysis of Zebrafish Locomotor Behaviour by Generalized Linear Mixed Models. Scientific Reports. 7:2937
Ganzen, L., Venkatraman, P., Pang, C.P., Leung, Y.F., Zhang, M. (2017) Utilizing Zebrafish Visual Behaviors in Drug Screening for Retinal Degeneration. International Journal of Molecular Sciences. 18(6):1185
Hensley, M.R., Chua, R.F., Leung, Y.F., Yang, J.Y., Zhang, G. (2016) Molecular Evolution of MDM1, a "Duplication-Resistant" Gene in Vertebrates. PLoS One. 11:e0163229
Hensley, M.R., Cui, Z., Chua, R.F., Simpson, S., Shammas, N.L., Yang, J.Y., Leung, Y.F., Zhang, G. (2016) Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development. Scientific Reports. 6:27816
Zhang, L., Xiang, L., Liu, Y., Venkatraman, P., Chong, L., Cho, J., Bonilla, S., Jin, Z.B., Pang, C.P., Ko, K.M., Ma, P., Zhang, M., Leung, Y.F. (2016) A Naturally-Derived Compound Schisandrin B Enhanced Light Sensation in the pde6c Zebrafish Model of Retinal Degeneration. PLoS One. 11:e0149663
Gao, Y., Zhang, G., Jelfs, B., Carmer, R., Venkatraman, P., Ghadami, M., Brown, S.A., Pang, C.P., Leung, Y.F., Chan, R.H., Zhang, M. (2016) Computational classification of different wild-type zebrafish strains based on their variation in light-induced locomotor response. Computers in Biology and Medicine. 69:1-9
Liu, Y., Carmer, R., Zhang, G., Venkatraman, P., Brown, S.A., Pang, C.P., Zhang, M., Ma, P., Leung, Y.F. (2015) Statistical Analysis of Zebrafish Locomotor Response. PLoS One. 10:e0139521
Bishop, B., Ho, K.K., Tyler, K., Smith, A., Bonilla, S., Leung, Y.F., Ogas, J. (2015) The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio. Biochimica et biophysica acta. Gene regulatory mechanisms. 1849(8):1040-50
Gao, Y., Chan, R.H., Chow, T.W., Zhang, L., Bonilla, S., Pang, C.P., Zhang, M., Leung, Y.F. (2014) A High-Throughput Zebrafish Screening Method for Visual Mutants by Light-Induced Locomotor Response. IEEE/ACM transactions on computational biology and bioinformatics. 11(4):693-701
Zhang, L., Bonilla, S., Zhang, Y., and Leung, Y.F. (2014) p35 promotes the differentiation of amacrine cell subtype in the zebrafish retina under the regulation of egr1. Developmental dynamics : an official publication of the American Association of Anatomists. 243(2):315-23
Zhang, L., Ma, P., Collery, R., Trowbridge, S., Zhang, M., Zhong, W., and Leung, Y.F. (2014) Expression profiling of the RPE in zebrafish smarca4 mutant revealed altered signals that potentially affect RPE and retinal differentiation. Molecular Vision. 20:56-72
Zhang, Y., Bonilla, S., Chong, L., and Leung, Y.F. (2013) Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas. Gene expression patterns : GEP. 13(5-6):177-82
Li, Z., Zhang, L., and Leung, Y.F. (2013) Use of the zebrafish model to study refractive error. Expert Review of Ophthalmology. 8(1):1-3
Zhang, L., Cho, J., Ptak, D., and Leung, Y.F. (2013) The Role of egr1 in Early Zebrafish Retinogenesis. PLoS One. 8(2):e56108
Zhang, L., Chong, L., Cho, J., Liao, P.C., Shen, F., and Leung, Y.F. (2012) Drug Screening to Treat Early-Onset Eye Diseases: Can Zebrafish Expedite the Discovery?. Asia-Pacific Journal of Ophthalmology. 1(6):374-383
Li, Z., Ptak, D., Zhang, L., Walls, E.K., Zhong, W., and Leung, Y.F. (2012) Phenylthiourea specifically reduces zebrafish eye size. PLoS One. 7(6):e40132
Zhang, Y., Yang, Y., Trujillo, C., Zhong, W., and Leung, Y.F. (2012) The Expression of irx7 in the Inner Nuclear Layer of Zebrafish Retina Is Essential for a Proper Retinal Development and Lamination. PLoS One. 7(4):e36145
Hensley, M.R., Emran, F., Bonilla, S., Zhang, L., Zhong, W., Grosu, P., Dowling, J.E., and Leung, Y.F. (2011) Cellular Expression of Smarca4 (Brg1)-regulated Genes in Zebrafish Retinas. BMC Developmental Biology. 11(1):45
Zhang, L., and Leung, Y.F. (2010) Microdissection of zebrafish embryonic eye tissues. Journal of visualized experiments : JoVE. (40)
Hensley, M.R., and Leung, Y.F. (2010) A Convenient Dry Feed for Raising Zebrafish Larvae. Zebrafish. 7(2):219-231
Leung, Y.F., Ma, P., Link, B.A., and Dowling, J.E. (2008) Factorial microarray analysis of zebrafish retinal development. Proceedings of the National Academy of Sciences of the United States of America. 105(35):12909-12914
Leung, Y.F., Ma, P., and Dowling, J.E. (2007) Gene expression profiling of zebrafish embryonic retinal pigment epithelium in vivo. Investigative ophthalmology & visual science. 48(2):881-890
Leung, Y.F., and Dowling, J.E. (2005) Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish. 2(4):269-283