ZFIN Lab: Heath Lab

ZFIN ID: ZDB-LAB-031030-1

Heath Lab

PI/Director:	Heath, Joan K.
Contact Person:	Heath, Joan K.
Email:	joan.heath@wehi.edu.au
URL:	http://www.wehi.edu.au/faculty_members/associate_professor_joan_heath
Address:	The Walter and Eliza Hall Institute of Medical Research 1G Royal Parade \| Parkville Melbourne \| Vic 3052 \| Australia
Country:	Australia
Phone:	(+613) 9345 2872
Fax:	(+613) 9347 0852
Line Designation:	None assigned

GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available

STATEMENT OF RESEARCH INTERESTS

Zebrafish intestinal epithelium
Genetic basis of colon cancer development
RNA splicing
RNA binding proteins
Gene expression

LAB MEMBERS

de Jong-Curtain, Tanya A. Post-Doc	Doggett, Karen Post-Doc	Geng, Fansuo Post-Doc
Jorissen, Robert Post-Doc	Love, Chris Post-Doc	Mierusznski, Stephen Post-Doc
Coates, Janine Research Staff	McPhee, Dora Research Staff	Blanch, Tyson Fish Facility Staff
Richards, Lysandra Fish Facility Staff	Morgan, Kimberly

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Baldera, D., Baxendale, S., van Hateren, N.J., Marzo, M., Glendenning, E., Geng, F.S., Yokoya, K., Knight, R.D., Whitfield, T.T. (2023) Enhancer trap lines with GFP driven by smad6b and frizzled1 regulatory sequences for the study of epithelial morphogenesis in the developing zebrafish inner ear. Journal of anatomy. 243(1):78-89

Morgan, K.J., Doggett, K., Geng, F., Mieruszynski, S., Whitehead, L., Smith, K.A., Hogan, B.M., Simons, C., Baillie, G.J., Molania, R., Papenfuss, A.T., Hall, T.E., Ober, E.A., Stainier, D.Y.R., Gong, Z., Heath, J.K. (2023) ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma. eLIFE. 12:

Koltowska, K., Okuda, K.S., Gloger, M., Rondon-Galeano, M., Mason, E., Xuan, J., Dudczig, S., Chen, H., Arnold, H., Skoczylas, R., Bower, N.I., Paterson, S., Lagendijk, A.K., Baillie, G.J., Leshchiner, I., Simons, C., Smith, K.A., Goessling, W., Heath, J.K., Pearson, R.B., Sanij, E., Schulte-Merker, S., Hogan, B.M. (2021) The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nature cell biology. 23:1136-1147

Geng, F.S., de la Calle-Mustienes, E., Gómez-Skarmeta, J.L., Lister, R., Bogdanovic, O. (2020) Depletion of Foxk transcription factors causes genome-wide transcriptional misregulation and developmental arrest in zebrafish embryos. microPublication. Biology. 2020:

Ross, S.E., Angeloni, A., Geng, F.S., de Mendoza, A., Bogdanovic, O. (2020) Developmental remodelling of non-CG methylation at satellite DNA repeats. Nucleic acids research. 48(22):12675-12688

Baell, J.B., Leaver, D.J., Hermans, S.J., Kelly, G.L., Brennan, M.S., Downer, N.L., Nguyen, N., Wichmann, J., McRae, H.M., Yang, Y., Cleary, B., Lagiakos, H.R., Mieruszynski, S., Pacini, G., Vanyai, H.K., Bergamasco, M.I., May, R.E., Davey, B.K., Morgan, K.J., Sealey, A.J., Wang, B., Zamudio, N., Wilcox, S., Garnham, A.L., Sheikh, B.N., Aubrey, B.J., Doggett, K., Chung, M.C., de Silva, M., Bentley, J., Pilling, P., Hattarki, M., Dolezal, O., Dennis, M.L., Falk, H., Ren, B., Charman, S.A., White, K.L., Rautela, J., Newbold, A., Hawkins, E.D., Johnstone, R.W., Huntington, N.D., Peat, T.S., Heath, J.K., Strasser, A., Parker, M.W., Smyth, G.K., Street, I.P., Monahan, B.J., Voss, A.K., Thomas, T. (2018) Inhibitors of histone acetyltransferases KAT6A/B induce senescence and arrest tumour growth. Nature. 560(7717):253-257

Miles, L.B., Darido, C., Kaslin, J., Heath, J.K., Jane, S.M., Dworkin, S. (2017) Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. Scientific Reports. 7:17607

Keightley, M.C., Carradice, D.P., Layton, J.E., Pase, L., Bertrand, J.Y., Wittig, J.G., Dakic, A., Badrock, A.P., Cole, N.J., Traver, D., Nutt, S.L., McCoey, J., Buckle, A.M., Heath, J.K., Lieschke, G.J. (2017) The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nature communications. 8:14911

Don, E.K., de Jong-Curtain, T.A., Doggett, K., Hall, T.E., Heng, B., Badrock, A.P., Winnick, C., Nicholson, G.A., Guillemin, G.J., Currie, P.D., Hesselson, D., Heath, J.K., Cole, N.J. (2016) Genetic basis of hindlimb loss in a naturally occurring vertebrate model. Biology Open. 5(3):359-66

Keightley, M.C., Markmiller, S., Love, C.G., Rasko, J.E., Lieschke, G.J., Heath, J.K. (2016) Experimental approaches to studying the nature and impact of splicing variation in zebrafish. Methods in cell biology. 135:259-88

Lobert, V.H., Mouradov, D., Heath, J.K. (2016) Focusing the Spotlight on the Zebrafish Intestine to Illuminate Mechanisms of Colorectal Cancer. Advances in experimental medicine and biology. 916:411-37

Rinkwitz, S., Geng, F., Manning, E., Suster, M., Kawakami, K., Becker, T.S. (2015) BAC transgenic zebrafish reveal hypothalamic enhancer activity around obesity associated SNP rs9939609 within the human FTO gene. Genesis (New York, N.Y. : 2000). 53(10):640-51

Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M. (2014) Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton. Mechanisms of Development. 133:77-90

Markmiller, S., Cloonan, N., Lardelli, R.M., Doggett, K., Keightley, M.C., Boglev, Y., Trotter, A.J., Ng, A.Y., Wilkins, S.J., Verkade, H., Ober, E.A., Field, H.A., Grimmond, S.M., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2014) Minor class splicing shapes the zebrafish transcriptome during development. Proceedings of the National Academy of Sciences of the United States of America. 111(8):3062-3067

Geng, F.S., Abbas, L., Baxendale, S., Holdsworth, C.J., Swanson, A.G., Slanchev, K., Hammerschmidt, M., Topczewski, J., and Whitfield, T.T. (2013) Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene. Development (Cambridge, England). 40(21):4362-4374

Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J. (2013) In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS letters. 587(14):2150-2157

Boglev, Y., Badrock, A.P., Trotter, A.J., Du, Q., Richardson, E.J., Parslow, A.C., Markmiller, S.J., Hall, N.E., de Jong-Curtain, T.A., Ng, A.Y., Verkade, H., Ober, E.A., Field, H.A., Shin, D., Shin, C.H., Hannan, K.M., Hannan, R.D., Pearson, R.B., Kim, S.H., Ess, K.C., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2013) Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis. PLoS Genetics. 9(2):e1002379

Pase, L., Layton, J.E., Wittmann, C., Ellett, F., Nowell, C.J., Reyes-Aldasoro, C.C., Varma, S., Rogers, K.L., Hall, C.J., Keightley, M.C., Crosier, P.S., Grabher, C., Heath, J.K., Renshaw, S.A., and Lieschke, G.J. (2012) Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish. Current biology : CB. 22(19):1818-1824

Dworkin, S., Darido, C., Georgy, S.R., Wilanowski, T., Srivastava, S., Ellett, F., Pase, L., Han, Y., Meng, A., Heath, J.K., Lieschke, G.J., and Jane, S.M. (2012) Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways. Development (Cambridge, England). 139(3):525-536

Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845

Cerveny, K.L., Cavodeassi, F., Turner, K.J., de Jong-Curtain, T.A., Heath, J.K., and Wilson, S.W. (2010) The zebrafish flotte lotte mutant reveals that the local retinal environment promotes the differentiation of proliferating precursors emerging from their stem cell niche. Development (Cambridge, England). 137(13):2107-2115

de Jong-Curtain, T.A., Parslow, A.C., Trotter, A.J., Hall, N.E., Verkade, H., Tabone, T., Christie, E.L., Crowhurst, M.O., Layton, J.E., Shepherd, I.T., Nixon, S.J., Parton, R.G., Zon, L.I., Stainier, D.Y., Lieschke, G.J., and Heath, J.K. (2009) Abnormal nuclear pore formation triggers apoptosis in the intestinal epithelium of elys-deficient zebrafish. Gastroenterology. 136(3):902-911

Trotter, A.J., Parslow, A.C., and Heath, J.K. (2009) Morphologic analysis of the zebrafish digestive system. Methods in molecular biology (Clifton, N.J.). 546:289-315

Mizoguchi, T., Verkade, H., Heath, J.K., Kuroiwa, A., and Kikuchi, Y. (2008) Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation. Development (Cambridge, England). 135(15):2521-2529

Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C. (2008) Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation. Developmental Biology. 314(1):12-22

Christie, E.L., Parslow, A.C., and Heath, J.K. (2008) Determination of mRNA and protein expression patterns in zebrafish. Methods in molecular biology (Clifton, N.J.). 469:253-272

Hogan, B.M., Verkade, H., Lieschke, G.J., and Heath, J.K. (2008) Manipulation of gene expression during zebrafish embryonic development using transient approaches. Methods in molecular biology (Clifton, N.J.). 469:273-300

Verkade, H., and Heath, J.K. (2008) Wnt signaling mediates diverse developmental processes in zebrafish. Methods in molecular biology (Clifton, N.J.). 469:225-251

Dworkin, S., Heath, J.K., Dejong-Curtain, T.A., Hogan, B.M., Lieschke, G.J., Malaterre, J., Ramsay, R.G., and Mantamadiotis, T. (2007) CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish. Developmental Biology. 307(1):127-141

Hogan, B.M., Layton, J.E., Pyati, U.J., Nutt, S.L., Hayman, J.W., Varma, S., Heath, J.K., Kimelman, D., and Lieschke, G.J. (2006) Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish. Current biology : CB. 16(5):506-511

Ng, A.N., de Jong-Curtain, T.A., Mawdsley, D.J., White, S.J., Shin, J., Appel, B., Dong, P.D., Stainier, D.Y., and Heath, J.K. (2005) Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis. Developmental Biology. 286(1):114-135

Hogan, B.M., Danks, J.A., Layton, J.E., Hall, N.E., Heath, J.K., and Lieschke, G.J. (2005) Duplicate zebrafish pth genes are expressed along the lateral line and in the central nervous system during embryogenesis. Endocrinology. 146(2):547-551

Onnebo, S.M., Condron, M.M., McPhee, D.O., Lieschke, G.J., and Ward, A.C. (2005) Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion. Experimental hematology. 33(2):182-188

Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J. (2004) Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm. Developmental Biology. 276(2):508-522

Mawdsley, D.J., Cooper, H.M., Hogan, B.M., Cody, S.H., Lieschke, G.J., and Heath, J.K. (2004) The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish. Developmental Biology. 269(1):302-315

Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., and Lieschke, G.J. (2003) The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish. Blood. 102(9):3238-3240

de Jong-Curtain, T.A. (2003) Genetic and morphological characterisation of the zebrafish intestinal mutant flotte lotte (flo). Ph.D. Thesis. :220p