ZFIN ID: ZDB-LAB-030320-1
Allende Lab
PI/Director: Allende, Miguel L.
Contact Person: Allende, Miguel L.
Email: allende@uchile.cl
URL: http://www.zebrafish.cl
Address: FONDAP Center for Genome Regulation Facultad de Ciencias Universidad de Chile Casilla 653 Santiago CHILE
Country: Chile
Phone: 56-2-978-7390
Fax: 56-2-276-3802
Line Designation: udc


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 1 genomic features


STATEMENT OF RESEARCH INTERESTS
We are interested in exploring different aspects of sensory cell development and regeneration in the zebrafish. As a model system for these studies, we favor the lateral line system, in which mechanosensory hair cells develop on the body surface within small organs called neuromasts. We have found that these cells are exquisitely sensitive to waterborne toxicants, including metals such as copper, but that hair cells can regenerate readily if the toxic agent is removed. We have also found that neuromast damage is followed by a potent innate immune response, which can be easily scored in transgenic fish expressing GFP in immune cells. The lateral line system has turned into a versatile screening tool, as oxidative stress, regeneration, inflammation and other biological assays can be carried out with the appropriate live markers in larvae.


LAB MEMBERS
Undurraga, Cristian Graduate Student d'Alençon, Claudia Graduate Student Espinoza, Florencio Technical Staff
Lafourcade, Catalina Technical Staff Silva, Juan Technical Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Paredes-Zúñiga, S., Morales, R.A., Muñoz-Sánchez, S., Muñoz-Montecinos, C., Parada, M., Tapia, K., Rubilar, C., Allende, M.L., Peña, O.A. (2017) CXCL12a/CXCR4b acts to retain neutrophils in caudal hematopoietic tissue and to antagonize recruitment to an injury site in the zebrafish larva. Immunogenetics.
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P. (2017) Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods. Journal of Microbiological Methods.
Pei, W., Huang, S.C., Xu, L., Pettie, K., Ceci, M.L., Sánchez, M., Allende, M.L., Burgess, S.M. (2016) Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish. Cell regeneration (London, England). 5:3
Carrillo, S.A., Anguita-Salinas, C., Peña, O.A., Morales, R.A., Muñoz-Sánchez, S., Muñoz-Montecinos, C., Paredes-Zúñiga, S., Tapia, K., Allende, M.L. (2016) Macrophage Recruitment Contributes to Regeneration of Mechanosensory Hair Cells in the Zebrafish Lateral Line. Journal of cellular biochemistry. 117(8):1880-9
Chávez, M.N., Schenck, T.L., Hopfner, U., Centeno-Cerdas, C., Somlai-Schweiger, I., Schwarz, C., Machens, H.G., Heikenwalder, M., Bono, M.R., Allende, M.L., Nickelsen, J., Egaña, J.T. (2015) Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration. Biomaterials. 75:25-36
Schenck, T.L., Hopfner, U., Chávez, M.N., Machens, H.G., Somlai-Schweiger, I., Giunta, R.E., Bohne, A.V., Nickelsen, J., Allende, M.L., Egaña, J.T. (2014) Photosynthetic biomaterials: a pathway towards autotrophic tissue engineering. Acta biomaterialia. 15:39-47
Ceci, M.L., Mardones-Krsulovic, C., Sánchez, M., Valdivia, L.E., Allende, M.L. (2014) Axon-Schwann cell interactions during peripheral nerve regeneration in zebrafish larvae. Neural Development. 9:22
Lange, S., Gonzalez, I., Pinto, M.P., Arce, M., Valenzuela, R., Aranda, E., Elliot, M., Alvarez, M., Henriquez, S., Velasquez, E.V., Orge, F., Oliva, B., Gonzalez, P., Villalon, M., Cautivo, K.M., Kalergis, A.M., Pereira, K., Mendoza, C., Saez, C., Kato, S., Cuello, M.A., Parborell, F., Irusta, G., Palma, V., Allende, M.L., Owen, G.I. (2014) Independent Anti-Angiogenic Capacities of Coagulation Factors X and Xa. Journal of Cellular Physiology. 229(11):1673-80
Moya-Díaz, J., Peña, O.A., Sánchez, M., Ureta, D.A., Reynaert, N.G., Anguita-Salinas, C., Marín, G., and Allende, M.L. (2014) Electroablation: a method for neurectomy and localized tissue injury. BMC Developmental Biology. 14:7
Armas, P., Margarit, E., Mouguelar, V.S., Allende, M.L., and Calcaterra, N.B. (2013) Beyond the Binding Site: In Vivo Identification of tbx2, smarca5 and wnt5b as Molecular Targets of CNBP during Embryonic Development. PLoS One. 8(5):e63234
Quezada, M., Alvarez, M., Peña, O.A., Henríquez, S., Alençon, C.A., Lange, S., Oliva, B., Owen, G.I., and Allende, M.L. (2013) Antiangiogenic, antimigratory and antiinflammatory effects of 2-methoxyestradiol in zebrafish larvae. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 157(2):141-149
Villegas, R., Martin, S.M., O'Donnell, K., Carrillo, S., Sagasti, A., and Allende, M.L. (2012) Dynamics of degeneration and regeneration in developing zebrafish peripheral axons reveals a requirement for extrinsic cell types. Neural Development. 7(1):19
Valdivia, L.E., Young, R.M., Hawkins, T.A., Stickney, H.L., Cavodeassi, F., Schwarz, Q., Pullin, L.M., Villegas, R., Moro, E., Argenton, F., Allende, M.L., and Wilson, S.W. (2011) Lef1-dependent Wnt/β-catenin signalling drives the proliferative engine that maintains tissue homeostasis during lateral line development. Development (Cambridge, England). 138(18):3931-3941
Hernandez, P.P., Undurraga, C., Gallardo, V.E., Mackenzie, N., Allende, M.L., and Reyes, A.E. (2011) Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae. Biological research. 44(1):7-15
Allende, M.L., Calcaterra, N.B., Vianna, M.R., and Zolessi, F.R. (2011) First Meeting of the Latin American Zebrafish Network. Zebrafish.
d'Alencon, C.A., Pena, O.A., Wittmann, C., Gallardo, V.E., Jones, R.A., Loosli, F., Liebel, U., Grabher, C., and Allende, M.L. (2010) A high-throughput chemically induced inflammation assay in zebrafish. BMC Biology. 8:151
Gallardo, V.E., Liang, J., Behra, M., Elkahloun, A., Villablanca, E.J., Russo, V., Allende, M.L., and Burgess, S.M. (2010) Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. BMC Developmental Biology. 10:120
Weiner, A.M., Allende, M.L., and Calcaterra, N.B. (2009) Zebrafish cnbp intron1 plays a fundamental role in controlling spatiotemporal gene expression during embryonic development. Journal of cellular biochemistry. 108(6):1364-1375
Pistocchi, A., Feijoo, C.G., Cabrera, P., Villablanca, E.J., Allende, M.L., and Cotelli, F. (2009) The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line. BMC Developmental Biology. 9:58
Feijoo, C.G., Sarrazin, A.F., Allende, M.L., and Glavic, A. (2009) Cystein-serine-rich nuclear protein 1, Axud1/Csrnp1, is essential for cephalic neural progenitor proliferation and survival in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 238(8):2034-2043
Nuñez, V.A., Sarrazin, A.F., Cubedo, N., Allende, M.L., Dambly-Chaudière, C., and Ghysen, A. (2009) Postembryonic development of the posterior lateral line in the zebrafish. Evolution & development. 11(4):391-404
Behra, M., Bradsher, J., Sougrat, R., Gallardo, V., Allende, M.L., and Burgess, S.M. (2009) Phoenix is required for mechanosensory hair cell regeneration in the zebrafish lateral line. PLoS Genetics. 5(4):e1000455
Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L. (2009) Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a. Molecular and Cellular Neuroscience. 40(3):328-337
Sabel, J.L., d'Alençon, C., O'Brien, E.K., Otterloo, E.V., Lutz, K., Cuykendall, T.N., Schutte, B.C., Houston, D.W., and Cornell, R.A. (2009) Maternal Interferon Regulatory Factor 6 is required for the differentiation of primary superficial epithelia in Danio and Xenopus embryos. Developmental Biology. 325(1):249-262
Olivari, F.A., Hernández, P.P., and Allende, M.L. (2008) Acute copper exposure induces oxidative stress and cell death in lateral line hair cells of zebrafish larvae. Brain research. 1244:1-12
Hernández, P.P., and Allende, M.L. (2008) Zebrafish (Danio rerio) as a model for studying the genetic basis of copper toxicity, deficiency, and metabolism. The American journal of clinical nutrition. 88(3):835S-839S
Bergeron, S.A., Milla, L.A., Villegas, R., Shen, M.C., Burgess, S.M., Allende, M.L., Karlstrom, R.O., and Palma, V. (2008) Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos. Genomics. 91(2):165-177
Weiner, A.M., Allende, M.L., Becker, T.S., and Calcaterra, N.B. (2007) CNBP mediates neural crest cell expansion by controlling cell proliferation and cell survival during rostral head development. Journal of cellular biochemistry. 102(6):1553-1570
Blanco, M.J., Barrallo-Gimeno, A., Acloque, H., Reyes, A.E., Tada, M., Allende, M.L., Mayor, R., and Nieto, M.A. (2007) Snail1a and Snail1b cooperate in the anterior migration of the axial mesendoderm in the zebrafish embryo. Development (Cambridge, England). 134(22):4073-4081
Villablanca, E.J., Pistocchi, A., Court, F.A., Cotelli, F., Bordignon, C., Allende, M.L., Traversari, C., and Russo, V. (2007) Abrogation of Prostaglandin E2/EP4 Signaling Impairs the Development of rag1+ Lymphoid Precursors in the Thymus of Zebrafish Embryos. Journal of immunology (Baltimore, Md. : 1950). 179(1):357-364
Hernandez, P.P., Olivari, F.A., Sarrazin, A.F., Sandoval, P.C., and Allende, M.L. (2007) Regeneration in zebrafish lateral line neuromasts: Expression of the neural progenitor cell marker sox2 and proliferation-dependent and-independent mechanisms of hair cell renewal. Developmental Neurobiology. 67(5):637-654
Rojas, D.A., Perez-Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., and Reyes, A.E. (2007) Cloning of hif-1alpha and hif-2alpha and mRNA expression pattern during development in zebrafish. Gene Expression Patterns. 7(3):339-345
Sarrazin, A.F., Villablanca, E.J., Nunez, V.A., Sandoval, P.C., Ghysen, A., and Allende, M.L. (2006) Proneural gene requirement for hair cell differentiation in the zebrafish lateral line. Developmental Biology. 295(2):534-545
Allende, M.L., Manzanares, M., Tena, J.J., Feijoo, C.G., and Gomez-Skarmeta, J.L. (2006) Cracking the genome's second code: Enhancer detection by combined phylogenetic footprinting and transgenic fish and frog embryos. Methods (San Diego, Calif.). 39(3):212-219
Cerda, G.A., Thomas, J.E., Allende, M.L., Karlstrom, R.O., and Palma, V. (2006) Electroporation of DNA, RNA, and morpholinos into zebrafish embryos. Methods (San Diego, Calif.). 39(3):207-211
Villablanca, E.J., Renucci, A., Sapede, D., Lec, V., Soubiran, F., Sandoval, P.C., Dambly-Chaudiere, C., Ghysen, A., and Allende, M.L. (2006) Control of cell migration in the zebrafish lateral line: Implication of the gene "Tumour-Associated Calcium Signal Transducer," tacstd. Developmental dynamics : an official publication of the American Association of Anatomists. 235(6):1578-1588
Hernandez, P.P., Moreno, V., Olivari, F.A., and Allende, M.L. (2006) Sub-lethal concentrations of waterborne copper are toxic to lateral line neuromasts in zebrafish (Danio rerio). Hearing Research. 213(1-2):1-10
de la Calle-Mustienes, E., Feijoo, C.G., Manzanares, M., Tena, J.J., Rodriguez-Seguel, E., Leticia, A., Allende, M.L., and Gomez-Skarmeta, J.L. (2005) A functional survey of the enhancer activity of conserved non-coding sequences from vertebrate Iroquois cluster gene deserts. Genome research. 15(8):1061-1072
Cavodeassi, F., Carreira-Barbosa, F., Young, R., Concha, M., Allende, M.L., Houart, C., Tada, M., and Wilson, S. (2005) Early Stages of Zebrafish Eye Formation Require the Coordinated Activity of Wnt11, Fz5, and the Wnt/Catenin Pathway. Neuron. 47(1):43-56
Armas, P., Cachero, S., Lombardo, V.A., Weiner, A., Allende, M.L., and Calcaterra, N.B. (2004) Zebrafish cellular nucleic acid-binding protein: gene structure and developmental behaviour. Gene. 337:151-161
Glavic, A., Maris Honoré, S., Gloria Feijóo, C., Bastidas, F., Allende, M.L., and Mayor, R. (2004) Role of BMP signaling and the homeoprotein iroquois in the specification of the cranial placodal field. Developmental Biology. 272(1):89-103
Feijoo, C.G., Manzanares, M., De La Calle-Mustienes, E., Gómez-Skarmeta, J.L., and Allende, M.L. (2004) The Irx gene family in zebrafish: genomic structure, evolution and initial characterization of irx5b. Development genes and evolution. 214(6):277-284
Mackenzie, N.C., Brito, M., Reyes, A.E., and Allende, M.L. (2004) Cloning, expression pattern and essentiality of the high-affinity copper transporter 1 (ctr1) gene in zebrafish. Gene. 328:113-120
O'Brien, E.K., d'Alencon, C., Bonde, G., Li, W., Schoenebeck, J., Allende, M.L., Gelb, B.D., Yelon, D., Eisen, J.S., and Cornell, R.A. (2004) Transcription factor Ap-2alpha is necessary for development of embryonic melanophores, autonomic neurons and pharyngeal skeleton in zebrafish. Developmental Biology. 265(1):246-261
Young, R., Reyes, A., and Allende, M. (2002) Expression and splice variant analysis of the zebrafish tcf4 transcription factor. Mechanisms of Development. 117(1-2):269-273
Young, R.M., Marty, S., Nakano, Y., Wang, H., Yamamoto, D., Lin, S., and Allende, M.L. (2002) Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 223(2):298-305
Semino, C.E. and Allende, M.L. (2000) Chitin oligosaccharides as candidate patterning agents in zebrafish embryogenesis. The International journal of developmental biology. 44(2):183-193
Becker, T.S., Burgess, S.M., Amsterdam, A.H., Allende, M.L., and Hopkins, N. (1998) not really finished is crucial for development of the zebrafish outer retina and encodes a transcription factor highly homologous to human Nuclear Respiratory Factor-1 and avian Initiation Binding Repressor. Development (Cambridge, England). 125:4369-4378
Semino, C.E., Allende, M.L., Bakkers, J., Sapink, H.P., and Robbins, P.P. (1998) Expression of Rhizobium Chitin Oligosaccharide Fucosyltransferase in zebrafish embryos disrupts normal development. Annals of the New York Academy of Sciences. 842:49-54
Amsterdam, A., Yoon, C., Allende, M., Becker, T., Kawakami, K., Burgess, S., Gaiano, N., and Hopkins, N. (1997) Retrovirus-mediated insertional mutagenesis in zebrafish and identification of a molecular marker for embryonic germ cells. Cold Spring Harbor symposia on quantitative biology. 62:437-450
Ekker, M., Akimenko, M.-A., Allende, M.L., Smith, R., Drouin, G., Langille, R.M., Weinberg, E.S., and Westerfield, M. (1997) Relationships among msx gene structure and function in zebrafish and other vertebrates. Mol. Biol. Evol.. 14:1008-1022
Allende, M.L., Amsterdam, A., Becker, T., Kawakami, K., Gaiano, N., and Hopkins, N. (1996) Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes and Development. 10(24):3141-3155
Odenthal, J., Haffter, P., Vogelsang, E., Brand, M., van Eeden, F.J., Furutani-Seiki, M., Granato, M., Hammerschmidt, M., Heisenberg, C.P., Jiang, Y.J., Kane, D.A., Kelsh, R.N., Mullins, M.C., Warga, R.M., Allende, M.L., Weinberg, E.S., and Nüsslein-Volhard, C. (1996) Mutations affecting the formation of the notochord in the zebrafish, Danio rerio. Development (Cambridge, England). 123:103-115
van Eeden, F.J., Granato, M., Schach, U., Brand, M., Furutani-Seiki-, M., Haffter, P., Hammerschmidt, M., Heisenberg, C.P., Jiang, Y.J., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., Warga, R.M., Allende, M.L., Weinberg, E.S., and Nüsslein-Volhard, C. (1996) Mutations affecting somite formation and patterning in the zebrafish, Danio rerio. Development (Cambridge, England). 123:153-164
Gaiano, N., Amsterdam, A., Kawakami, K., Allende, M., Becker, T., and Hopkins, N. (1996) Insertional mutagenesis and rapid cloning of essential genes in zebrafish. Nature. 383(6603):829-832
Gaiano, N., Allende, M., Amsterdam, A., Kawakami, K., and Hopkins, N. (1996) Highly efficient germ-line transmission of proviral insertions in zebrafish. Proc. Natl. Acad. Sci. USA. 93(15):7777-7782
Weinberg, E.S., Allende, M.L., Kelly, C.S., Abdelhamid, A., Murakami, T., Andermann, P., Doerre, O.G., Grunwald, D.J., and Riggleman, B. (1996) Developmental regulation of zebrafish MyoD in wild-type, no-tail and spadetail embryos. Development (Cambridge, England). 122:271-280
Li, Y., Allende, M.L., Finkelstein, R., and Weinberg, E.S. (1994) Expression of two zebrafish orthodenticle-related genes in the embryonic brain. Mechanisms of Development. 48(3):229-244
Allende, M.L. (1994) Characterization and embryonic expression of two zebrafish Achaete-Scute homolog genes. Ph.D. Thesis.
Allende, M.L. and Weinberg, E.S. (1994) The expression pattern of two zebrafish achaete-scute homolog (ash) genes is altered in the embryonic brain of the cyclops mutant. Developmental Biology. 166:509-530
Daniotti, J.L., Allende, M.L., Weinberg, E.S., and Allende, J.E. (1994) Cloning and expression of genes coding for protein kinase CK2 alpha and beta subunits in zebrafish (Danio rerio). Cellular & molecular biology research. 40:431-439