Liu et al., 2017 - High-throughput imaging of zebrafish embryos using a linear-CCD-based flow imaging system. Biomedical Optics Express   8:5651-5662 Full text @ Biomed. Opt. Express

Fig. 3

(a) Original image of zebrafish embryos obtained by Lc-FIS. The white arrow indicates the flow direction. (b) Preprocessed image after substracting the background.

Fig. 4 Segmentation of zebrafish embryos with chorion using Hough-transformation circle detection algorithm. (a–c) Zebrafish embryos at different development stages: 3, 13, and 21.5 hpf, respectively. (d–f) Detailed structure of yolk, head, and tail of zebrafish embryos. H, head; T, tail; Y, yolk. The scale bars in (a)–(c) represent 1 mm, and those in (d)–(f) represent 0.3 mm.

Fig. 5 Discrimination of live and dead embryos by Lc-FIS. (a) Normal and (b) dead embryos (4 hpf) detected using the Hough-transformation algorithm. (c) and (d) Intensity distributions within the circle of the live and dead embryos, respectively. (e) Cumulative mortality rate of zebrafish embryos at temperatures of 24°C, 26°C, 28°C, 30°C, and 32°C.

Fig. 6

Flowchart of segmentation for zebrafish embryos without chorion.

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express