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Fig. 5 Discrimination of live and dead embryos by Lc-FIS. (a) Normal and (b) dead embryos (4 hpf) detected using the Hough-transformation algorithm. (c) and (d) Intensity distributions within the circle of the live and dead embryos, respectively. (e) Cumulative mortality rate of zebrafish embryos at temperatures of 24°C, 26°C, 28°C, 30°C, and 32°C.
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