|ZFIN ID: ZDB-PUB-991201-72|
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Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops
Varga, Z.M., Wegner, J., and Westerfield, M.
|Source:||Development (Cambridge, England) 126: 5533-5546 (Journal)|
|Registered Authors:||Varga, Zoltan M., Wegner, Jeremy, Westerfield, Monte|
|Keywords:||fate map; anterior neural plate; cyclopia; holoprosencephaly; zebrafish; Danio rerio; gene expression; morphogenesis; odd-paired-like (opl)|
Varga, Z.M., Wegner, J., and Westerfield, M. (1999) Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops. Development (Cambridge, England). 126:5533-5546.
ABSTRACTA currently favored hypothesis postulates that a single field of cells in the neural plate forms bilateral retinas. To learn how retinal precursors segregate, we followed individual labeled neural plate cells in zebrafish. In the late gastrula, a single field of odd-paired-like-expressing cells contributed to both retinas, bordered posteriorly by diencephalic precursors expressing mariposa. Median mariposa-expressing cells moved anteriorly, separating the eyes, and formed ventral anterior diencephalon, the presumptive hypothalamus. In cyclops mutants, corresponding cells failed to move anteriorly, a ventral diencephalon never formed, and the eyes remained fused. Ablation of the region containing these cells induced cyclopia in wild types. Our results indicate that movement of a median subpopulation of diencephalic precursors separates retinal precursors into left and right eyes. Wild-type cyclops gene function is required for these morphogenetic movements.