Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops
- Varga, Z.M., Wegner, J., and Westerfield, M.
- Development (Cambridge, England) 126: 5533-5546 (Journal)
- Registered Authors
- Varga, Zoltan M., Wegner, Jeremy, Westerfield, Monte
- fate map; anterior neural plate; cyclopia; holoprosencephaly; zebrafish; Danio rerio; gene expression; morphogenesis; odd-paired-like (opl)
- MeSH Terms
- Gene Expression Regulation, Developmental
- Stem Cells
- 10572031 Full text @ Development
Varga, Z.M., Wegner, J., and Westerfield, M. (1999) Anterior movement of ventral diencephalic precursors separates the primordial eye field in the neural plate and requires cyclops. Development (Cambridge, England). 126:5533-5546.
A currently favored hypothesis postulates that a single field of cells in the neural plate forms bilateral retinas. To learn how retinal precursors segregate, we followed individual labeled neural plate cells in zebrafish. In the late gastrula, a single field of odd-paired-like-expressing cells contributed to both retinas, bordered posteriorly by diencephalic precursors expressing mariposa. Median mariposa-expressing cells moved anteriorly, separating the eyes, and formed ventral anterior diencephalon, the presumptive hypothalamus. In cyclops mutants, corresponding cells failed to move anteriorly, a ventral diencephalon never formed, and the eyes remained fused. Ablation of the region containing these cells induced cyclopia in wild types. Our results indicate that movement of a median subpopulation of diencephalic precursors separates retinal precursors into left and right eyes. Wild-type cyclops gene function is required for these morphogenetic movements.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes