PUBLICATION
Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish
- Authors
- Mizuno, T., Yamaha, E., Kuroiwa, A., and Takeda, H.
- ID
- ZDB-PUB-990628-29
- Date
- 1999
- Source
- Mechanisms of Development 81(1-2): 51-63 (Journal)
- Registered Authors
- Mizuno, Toshiro, Takeda, Hiroyuki
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastomeres/physiology
- Body Patterning
- Fetal Proteins/metabolism
- Gene Expression Regulation, Developmental
- Goosecoid Protein
- Homeodomain Proteins/metabolism
- In Situ Hybridization
- Models, Biological
- Molecular Sequence Data
- Repressor Proteins*
- T-Box Domain Proteins*
- Time Factors
- Transcription Factors*
- Yolk Sac/physiology*
- Zebrafish/embryology*
- Zebrafish Proteins*
- PubMed
- 10330484 Full text @ Mech. Dev.
Citation
Mizuno, T., Yamaha, E., Kuroiwa, A., and Takeda, H. (1999) Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish. Mechanisms of Development. 81(1-2):51-63.
Abstract
To examine the nature of cytoplasm determinants for dorsal specification in zebrafish, we have developed a method in which we remove the vegetal yolk hemisphere of early fertilized eggs (vegetal removed embryos). When the vegetal yolk mass was removed at the 1-cell stage, the embryos frequently exhibited typical ventralized phenotypes: no axial structures developed. The frequency of dorsal defects decreased when the operation was performed at later stages. Furthermore, the yolk cell obtained from the vegetal-removed embryos lost the ability to induce goosecoid in normal blastomeres while the normal yolk cell frequently did so in normal and vegetal-removed embryos. These results suggested that the vegetal yolk cell mass contains the dorsal determinants, and that the dorsal-inducing ability of the yolk cell is dependent on the determinants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping