ZFIN ID: ZDB-PUB-990628-29
Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish
Mizuno, T., Yamaha, E., Kuroiwa, A., and Takeda, H.
Date: 1999
Source: Mechanisms of Development   81(1-2): 51-63 (Journal)
Registered Authors: Mizuno, Toshiro, Takeda, Hiroyuki
Keywords: none
MeSH Terms:
  • Animals
  • Blastomeres/physiology
  • Body Patterning
  • Fetal Proteins/metabolism
  • Gene Expression Regulation, Developmental
  • Goosecoid Protein
  • Homeodomain Proteins/metabolism
  • In Situ Hybridization
  • Models, Biological
  • Molecular Sequence Data
  • Repressor Proteins*
  • T-Box Domain Proteins*
  • Time Factors
  • Transcription Factors*
  • Yolk Sac/physiology*
  • Zebrafish/embryology*
  • Zebrafish Proteins*
PubMed: 10330484 Full text @ Mech. Dev.
To examine the nature of cytoplasm determinants for dorsal specification in zebrafish, we have developed a method in which we remove the vegetal yolk hemisphere of early fertilized eggs (vegetal removed embryos). When the vegetal yolk mass was removed at the 1-cell stage, the embryos frequently exhibited typical ventralized phenotypes: no axial structures developed. The frequency of dorsal defects decreased when the operation was performed at later stages. Furthermore, the yolk cell obtained from the vegetal-removed embryos lost the ability to induce goosecoid in normal blastomeres while the normal yolk cell frequently did so in normal and vegetal-removed embryos. These results suggested that the vegetal yolk cell mass contains the dorsal determinants, and that the dorsal-inducing ability of the yolk cell is dependent on the determinants.