|ZFIN ID: ZDB-PUB-990628-29|
Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish
Mizuno, T., Yamaha, E., Kuroiwa, A., and Takeda, H.
|Source:||Mechanisms of Development 81(1-2): 51-63 (Journal)|
|Registered Authors:||Mizuno, Toshiro, Takeda, Hiroyuki|
|PubMed:||10330484 Full text @ Mech. Dev.|
Mizuno, T., Yamaha, E., Kuroiwa, A., and Takeda, H. (1999) Removal of vegetal yolk causes dorsal deficencies and impairs dorsal-inducing ability of the yolk cell in zebrafish. Mechanisms of Development. 81(1-2):51-63.
ABSTRACTTo examine the nature of cytoplasm determinants for dorsal specification in zebrafish, we have developed a method in which we remove the vegetal yolk hemisphere of early fertilized eggs (vegetal removed embryos). When the vegetal yolk mass was removed at the 1-cell stage, the embryos frequently exhibited typical ventralized phenotypes: no axial structures developed. The frequency of dorsal defects decreased when the operation was performed at later stages. Furthermore, the yolk cell obtained from the vegetal-removed embryos lost the ability to induce goosecoid in normal blastomeres while the normal yolk cell frequently did so in normal and vegetal-removed embryos. These results suggested that the vegetal yolk cell mass contains the dorsal determinants, and that the dorsal-inducing ability of the yolk cell is dependent on the determinants.
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