PUBLICATION
Generation of a zebrafish P1 artificial chromosome library
- Authors
- Amemiya, C.T. and Zon, L.I.
- ID
- ZDB-PUB-990624-66
- Date
- 1999
- Source
- Genomics 58(2): 211-213 (Journal)
- Registered Authors
- Amemiya, Chris, Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Molecular Biology/methods*
- Animals
- Chromosomes*
- Gene Library*
- Zebrafish/genetics*
- PubMed
- 10366454 Full text @ Genomics
Citation
Amemiya, C.T. and Zon, L.I. (1999) Generation of a zebrafish P1 artificial chromosome library. Genomics. 58(2):211-213.
Abstract
We have constructed a genomic P1 artificial chromosome library from the zebrafish. The library has been arrayed and archived in two hundred seventy-one 384-well microtiter dishes. It encompasses four to five genome equivalents with an average insert size of approximately 115 kb and is readily accessible to the scientific community. The library has been used by numerous investigators in the community and shown to be a useful reagent for chromosomal walking and positional cloning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping