PUBLICATION

Generation of a zebrafish P1 artificial chromosome library

Authors
Amemiya, C.T. and Zon, L.I.
ID
ZDB-PUB-990624-66
Date
1999
Source
Genomics   58(2): 211-213 (Journal)
Registered Authors
Amemiya, Chris, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Chromosomes*
  • Gene Library*
  • Molecular Biology/methods*
  • Zebrafish/genetics*
PubMed
10366454 Full text @ Genomics
Abstract
We have constructed a genomic P1 artificial chromosome library from the zebrafish. The library has been arrayed and archived in two hundred seventy-one 384-well microtiter dishes. It encompasses four to five genome equivalents with an average insert size of approximately 115 kb and is readily accessible to the scientific community. The library has been used by numerous investigators in the community and shown to be a useful reagent for chromosomal walking and positional cloning.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes