PUBLICATION

A novel homeobox gene, dharma, can induce the organizer in a non-cell-autonomous manner

Authors
Yamanaka, Y., Mizuno, T., Sasai, Y., Kishi, M., Takeda, H., Kim, C.-H., Hibi, M., and Hirano, T.
ID
ZDB-PUB-980812-1
Date
1998
Source
Genes & Development   12: 2345-2353 (Journal)
Registered Authors
Hibi, Masahiko, Kim, Cheol-Hee, Mizuno, Toshiro, Takeda, Hiroyuki, Yamanaka, Yojiro
Keywords
organizer; Nieuwkoop center; dharma; zebrafish; yolk syncytial layer
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Body Patterning/genetics*
  • Cloning, Molecular
  • Embryonic Induction/genetics
  • Gene Expression Regulation, Developmental
  • Genes, Homeobox*
  • Homeodomain Proteins/genetics*
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Proteins/genetics
  • RNA, Messenger/genetics
  • Xenopus/embryology
  • Xenopus/genetics
  • Xenopus Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed
9694799 Full text @ Genes & Dev.
Abstract
The formation of Spemann organizer is one of the most important steps in dorsoventral axis determination in vertebrate development. However, whether the organizer forms autonomously or is induced non-cell- autonomously is controversial. In this report we have isolated a novel zebrafish homeobox gene, dharma, capable of inducing the organizer ectopically. The expression of dharma was first detected in several blastomeres at one side of the margin soon after the mid-blastula transition and continued in the dorsal side of the yolk syncytial layer (YSL) under the embryonic shield, the zebrafish organizer, until the onset of gastrulation. Furthermore, dharma expressed in the YSL induced the organizer in a non-cell-autonomous manner. These results provided the first identification of a zygotic gene to be implicated in the formation of an organizer-inducing center.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes