ZFIN ID: ZDB-PUB-970116-4
Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction
Fritz, A., Rozowski, M., Walker, C., and Westerfield, M.
Date: 1996
Source: Genetics 144(4): 1735-1745 (Journal)
Registered Authors: Fritz, Andreas, Rozowski, Marion, Walker, Charline, Westerfield, Monte
Keywords: none
MeSH Terms: Animals; DNA Primers; Gamma Rays; Mutation/radiation effects*; Polymerase Chain Reaction/methods* (all 6) expand
PubMed: 8978059
FIGURES   (current status)
ABSTRACT
The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.
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