PUBLICATION
Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction
- Authors
- Fritz, A., Rozowski, M., Walker, C., and Westerfield, M.
- ID
- ZDB-PUB-970116-4
- Date
- 1996
- Source
- Genetics 144(4): 1735-1745 (Journal)
- Registered Authors
- Fritz, Andreas, Rozowski, Marion, Walker, Charline, Westerfield, Monte
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Primers
- Gamma Rays
- Mutation/radiation effects*
- Polymerase Chain Reaction/methods*
- Zebrafish/genetics*
- PubMed
- 8978059 Full text @ Genetics
Citation
Fritz, A., Rozowski, M., Walker, C., and Westerfield, M. (1996) Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction. Genetics. 144(4):1735-1745.
Abstract
The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping