PUBLICATION

Cloning and expression of genes coding for protein kinase CK2 alpha and beta subunits in zebrafish (Danio rerio)

Authors
Daniotti, J.L., Allende, M.L., Weinberg, E.S., and Allende, J.E.
ID
ZDB-PUB-961104-7
Date
1994
Source
Cellular & molecular biology research   40: 431-439 (Journal)
Registered Authors
Allende, Jorge E., Allende, Miguel L., Weinberg, Eric
Keywords
none
MeSH Terms
  • Vertebrates/genetics
  • Vertebrates/metabolism
  • Gene Expression Regulation, Developmental*
  • Embryo, Nonmammalian/enzymology
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Protein Serine-Threonine Kinases/biosynthesis
  • Protein Serine-Threonine Kinases/genetics*
  • Amino Acid Sequence
  • Nerve Tissue Proteins/biosynthesis
  • Nerve Tissue Proteins/genetics
  • In Situ Hybridization
  • Species Specificity
  • Sequence Alignment
  • Drosophila melanogaster/enzymology
  • Drosophila melanogaster/genetics
  • Genes*
  • Cloning, Molecular
  • Casein Kinase II
  • Base Sequence
  • Saccharomyces cerevisiae/enzymology
  • Saccharomyces cerevisiae/genetics
  • Caenorhabditis elegans/enzymology
  • Caenorhabditis elegans/genetics
  • Enzyme Induction
  • Animals
  • Fungal Proteins/chemistry
  • RNA, Messenger/analysis
  • DNA, Complementary/genetics
  • Helminth Proteins/chemistry
  • Nervous System/embryology
  • Nervous System/enzymology
PubMed
7735317
Abstract
cDNA clones coding for the alpha and beta subunits of protein kinase 2 (CK2) in zebrafish (Danio rerio) have been isolated. Sequencing of the cDNA clones has demonstrated that one contains the complete coding sequence for the beta subunit of CK2 while the alpha clone is truncated and lacks 183 nucleotides of the 5' coding region. Comparison of the deduced amino acid sequences shows an extremely high degree of evolutionary sequence conservation of these two proteins. Northern analysis of the mRNAs coding for the alpha subunit indicates that this messenger is present in 1 h embryos as a 3.6 Kb and a 1.9 Kb species, both of which decrease in 24-h embryos. In the case of beta, the major mRNA species of approximately 1.7 Kb maintains its level during the period of embryogenesis studied. In situ hybridization of early embryos, using antisense RNAs against alpha and beta mRNAs demonstrates temporal and tissue specific expression patterns. The alpha mRNA decreases after blastula, when it is evenly distributed. The beta mRNA is maintained at high levels between 4 and 24 h of development, showing in 18 h embryos a higher concentration in the developing neural tube and in the embryonic optic and otic vesicles.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping