PUBLICATION
The zebrafish ultraviolet cone opsin reported previously is expressed in rods
- Authors
- Raymond, P.A., Barthel, L.K., and Stenkamp, D.L.
- ID
- ZDB-PUB-961014-924
- Date
- 1996
- Source
- Investigative ophthalmology & visual science 37(5): 948-950 (Journal)
- Registered Authors
- Barthel, Linda, Raymond, Pamela, Stenkamp, Deborah L.
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- DNA, Complementary/analysis
- DNA, Complementary/chemistry
- Gene Expression
- Goldfish
- In Situ Hybridization
- Molecular Sequence Data
- RNA Probes
- Retinal Cone Photoreceptor Cells/chemistry*
- Retinal Rod Photoreceptor Cells/chemistry*
- Rod Opsins/analysis*
- Rod Opsins/genetics
- Ultraviolet Rays
- Zebrafish*
- PubMed
- 8603882
Citation
Raymond, P.A., Barthel, L.K., and Stenkamp, D.L. (1996) The zebrafish ultraviolet cone opsin reported previously is expressed in rods. Investigative ophthalmology & visual science. 37(5):948-950.
Abstract
PURPOSE: To examine expression of the zebrafish ultraviolet cone opsin pigment in goldfish and zebrafish retinas. METHODS: Digoxigenin-labeled cRNA probes were prepared by run-off transcription from plasmids containing cDNAs for zebrafish ultraviolet opsin, goldfish ultraviolet cone opsin, and goldfish rod opsin. Probes were hybridized to cryosections of retina and visualized with immunocytochemistry. RESULTS: The zebrafish ultraviolet opsin probe hybridized selectively to rod photoreceptors, but not to ultraviolet cones or any other cone type, in both zebrafish and goldfish retinas, and the pattern of expression was identical to that of the goldfish rod opsin probe. The goldfish ultraviolet opsin, in contrast, hybridized to ultraviolet cone photoreceptors in both goldfish and zebrafish. CONCLUSIONS: The cDNA previously identified by Robinson et al as zebrafish ultraviolet opsin is not a cone opsin but is likely to be a rod opsin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping