ZFIN ID: ZDB-PUB-961014-888
A genetic linkage map for the zebrafish
Postlethwait, J., Johnson, S., Midson, C.N., Talbot, W.S., Gates, M., Ballenger, E.W., Africa, D., Andrews, R., Carl, T., Eisen, J.S., Horne, S., Kimmel, C.B., Hutchinson, M., Johnson, M., and Rodriguez, A.
Date: 1994
Source: Science (New York, N.Y.) 264: 699-703 (Journal)
Registered Authors: Eisen, Judith S., Gates, Michael A., Horne-Badovinac, Sally, Johnson, Stephen L., Kimmel, Charles B., Montgomery, Rebecca, Postlethwait, John H., Talbot, William S.
Keywords: none
MeSH Terms: Animals; Chromosome Mapping*; Cloning, Molecular; Female; Genetic Markers (all 13) expand
PubMed: 8171321 Full text @ Science
ABSTRACT
To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish Danio (Brachydanio) rerio. The map consists of 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval of 5.8 centimorgans. Strategies that exploit the advantages of haploid genetics and RAPD markers were developed that quickly mapped lethal and visible mutations and that placed cloned genes on the map. This map is useful for the position-based cloning of mutant genes, the characterization of chromosome rearrangements, and the investigation of evolution in vertebrate genomes.
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