ZFIN ID: ZDB-PUB-961014-498
Identification of RAPD primers that reveal extensive polymorphisms between laboratory strains of zebrafish
Johnson, S.L., Midson, C.N., Ballenger, E.W., and Postlethwait, J.H.
Date: 1994
Source: Genomics 19: 152-156 (Journal)
Registered Authors: Johnson, Stephen L., Postlethwait, John H.
Keywords: none
MeSH Terms: Animals; Base Sequence; Chromosome Mapping; DNA Primers*; Genetic Markers* (all 10) expand
PubMed: 8188217 Full text @ Genomics
ABSTRACT
The zebrafish has recently emerged as a useful system for understanding vertebrate developmental genetics, despite the lack of a linkage map. To identify DNA-based genetic polymorphisms for constructing a genetic map, we have screened a collection of RAPD primers for their utility in identifying genetic polymorphisms between two laboratory strains of zebrafish. Here, we report 116 primers that identify 721 strain-specific genetic markers and show how they can be used in haploid genetics of zebrafish.
ADDITIONAL INFORMATION