PUBLICATION

Cell-autonomous shift from axial to paraxial mesodermal development in zebrafish floating head mutants

Authors
Halpern, M.E., Thisse, C., Ho, R.K., Thisse, B., Riggleman, B., Trevarrow, B., Weinberg, E.S., Postlethwait, J.H., and Kimmel, C.B.
ID
ZDB-PUB-961014-389
Date
1995
Source
Development (Cambridge, England)   121: 4257-4264 (Journal)
Registered Authors
Halpern, Marnie E., Ho, Robert K., Kimmel, Charles B., Postlethwait, John H., Riggleman, Bob, Thisse, Bernard, Thisse, Christine, Trevarrow, Bill, Weinberg, Eric
Keywords
none
MeSH Terms
  • Animals
  • Cell Lineage
  • Gastrula/physiology
  • Gene Expression Regulation, Developmental*
  • Genes*
  • In Situ Hybridization
  • Mesoderm/physiology*
  • Morphogenesis
  • Muscles/embryology
  • Mutation
  • Notochord/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
8575325
Abstract
Zebrafish floating head mutant embryos lack notochord and develop somitic muscle in its place. This may result from incorrect specification of the notochord domain at gastrulation, or from respecification of notochord progenitors to form muscle. In genetic mosaics, floating head acts cell autonomously. Transplanted wild-type cells differentiate into notochord in mutant hosts; however, cells from floating head mutant donors produce muscle rather than notochord in wild-type hosts. Consistent with respecification, markers of axial mesoderm are initially expressed in floating head mutant gastrulas, but expression does not persist. Axial cells also inappropriately express markers of paraxial mesoderm. Thus, single cells in the mutant midline transiently co-express genes that are normally specific to either axial or paraxial mesoderm. Since floating head mutants produce some floor plate in the ventral neural tube, midline mesoderm may also retain early signaling capabilities. Our results suggest that wild- type floating head provides an essential step in maintaining, rather than initiating, development of notochord-forming axial mesoderm.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes