PUBLICATION
Identification of two families of satellite-like repetitive DNA sequences from the zebrafish (Brachydanio rerio)
- Authors
- Ekker, M., Fritz, A., and Westerfield, M.
- ID
- ZDB-PUB-961014-281
- Date
- 1992
- Source
- Genomics 13: 1169-1173 (Journal)
- Registered Authors
- Ekker, Marc, Fritz, Andreas, Westerfield, Monte
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA, Satellite/genetics*
- Genomic Library
- Molecular Sequence Data
- Repetitive Sequences, Nucleic Acid*
- Zebrafish/genetics*
- PubMed
- 1339388 Full text @ Genomics
Citation
Ekker, M., Fritz, A., and Westerfield, M. (1992) Identification of two families of satellite-like repetitive DNA sequences from the zebrafish (Brachydanio rerio). Genomics. 13:1169-1173.
Abstract
To further our understanding of the structure and organization of the zebrafish genome, we have undertaken the analysis of highly and middle-repetitive DNA sequences. We have cloned and sequenced two families of tandemly repeated DNA fragments. The monomer units of the Type I satellite-like sequence are 186 bp long, A+T-rich (65%), and exhibit a high degree of sequence conservation. The Type I satellite-like sequence constitutes 8% of the zebrafish genome, or approximately 8 x 10(5) copies per haploid genome. Southern analysis of genomic DNA, digested with several restriction endonucleases, shows a ladder of hybridizing bands, consistent with a tandem array, and suggests longer range periodic variations in the sequence of the tandem repeats. The Type II satellite has a monomer length of 165 bp, is also A+T-rich (68%), and constitutes 0.2% of the zebrafish genome (22,000 copies per haploid genome). Southern analysis reveals a complex pattern rather than a ladder of regularly spaced hybridizing bands.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping