PUBLICATION
Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish
- Authors
- Zheng, N., Wang, X., Zhang, Y., Hua, J., Zhu, B., Zhou, Y., Xu, Z., Luo, L., Han, J., Yang, L., Zhou, B.
- ID
- ZDB-PUB-240503-5
- Date
- 2024
- Source
- Environmental science & technology 58(19): 8251-8263 (Journal)
- Registered Authors
- Yang, LiHua, Zhou, BingSheng
- Keywords
- 1,2-bis(2,4,6-tribromophenoxy)ethane, male reproductive toxicity, mitochondrial impairment, phosphoproteomics, sperm quality
- MeSH Terms
-
- Animals
- Female
- Flame Retardants/toxicity
- Male
- Mitochondria/drug effects
- Mitochondria/metabolism
- Reproduction*/drug effects
- Spermatozoa/drug effects
- Testis/drug effects
- Testis/metabolism
- Zebrafish*
- PubMed
- 38695612 Full text @ Env. Sci. Tech.
Citation
Zheng, N., Wang, X., Zhang, Y., Hua, J., Zhu, B., Zhou, Y., Xu, Z., Luo, L., Han, J., Yang, L., Zhou, B. (2024) Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish. Environmental science & technology. 58(19):8251-8263.
Abstract
The novel brominated flame retardant, 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), has increasingly been detected in environmental and biota samples. However, limited information is available regarding its toxicity, especially at environmentally relevant concentrations. In the present study, adult male zebrafish were exposed to varying concentrations of BTBPE (0, 0.01, 0.1, 1, and 10 μg/L) for 28 days. The results demonstrated underperformance in mating behavior and reproductive success of male zebrafish when paired with unexposed females. Additionally, a decline in sperm quality was confirmed in BTBPE-exposed male zebrafish, characterized by decreased total motility, decreased progressive motility, and increased morphological malformations. To elucidate the underlying mechanism, an integrated proteomic and phosphoproteomic analysis was performed, revealing a predominant impact on mitochondrial functions at the protein level and a universal response across different cellular compartments at the phosphorylation level. Ultrastructural damage, increased expression of apoptosis-inducing factor, and disordered respiratory chain confirmed the involvement of mitochondrial impairment in zebrafish testes. These findings not only provide valuable insights for future evaluations of the potential risks posed by BTBPE and similar chemicals but also underscore the need for further research into the impact of mitochondrial dysfunction on reproductive health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping