PUBLICATION
Transgenic tools targeting the basal ganglia reveal both evolutionary conservation and specialization of neural circuits in zebrafish
- Authors
- Tanimoto, Y., Kakinuma, H., Aoki, R., Shiraki, T., Higashijima, S.I., Okamoto, H.
- ID
- ZDB-PUB-240315-1
- Date
- 2024
- Source
- Cell Reports 43(3): 113916 (Journal)
- Registered Authors
- Higashijima, Shin-ichi, Kakinuma, Hisaya, Okamoto, Hitoshi, Shiraki, Toshiyuki, Tanimoto, Yuki
- Keywords
- CP: Cell biology, CP: Neuroscience, basal ganglia, cortico-basal ganglia circuit, direct pathway, entopeduncular nucleus, indirect pathway, pallidum, striatum, telencephalon, transgenic zebrafish
- Datasets
- GEO:GSE254980
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Basal Ganglia*/physiology
- Corpus Striatum
- Globus Pallidus/physiology
- Mammals
- Neural Pathways/physiology
- Zebrafish*/genetics
- PubMed
- 38484735 Full text @ Cell Rep.
Citation
Tanimoto, Y., Kakinuma, H., Aoki, R., Shiraki, T., Higashijima, S.I., Okamoto, H. (2024) Transgenic tools targeting the basal ganglia reveal both evolutionary conservation and specialization of neural circuits in zebrafish. Cell Reports. 43(3):113916.
Abstract
The cortico-basal ganglia circuit mediates decision making. Here, we generated transgenic tools for adult zebrafish targeting specific subpopulations of the components of this circuit and utilized them to identify evolutionary homologs of the mammalian direct- and indirect-pathway striatal neurons, which respectively project to the homologs of the internal and external segment of the globus pallidus (dorsal entopeduncular nucleus [dEN] and lateral nucleus of the ventral telencephalic area [Vl]) as in mammals. Unlike in mammals, the Vl mainly projects to the dEN directly, not by way of the subthalamic nucleus. Further single-cell RNA sequencing analysis reveals two pallidal output pathways: a major shortcut pathway directly connecting the dEN with the pallium and the evolutionarily conserved closed loop by way of the thalamus. Our resources and circuit map provide the common basis for the functional study of the basal ganglia in a small and optically tractable zebrafish brain for the comprehensive mechanistic understanding of the cortico-basal ganglia circuit.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping