PUBLICATION
Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation
- Authors
- Kirchberger, S., Shoeb, M.R., Lazic, D., Wenninger-Weinzierl, A., Fischer, K., Shaw, L.E., Nogueira, F., Rifatbegovic, F., Bozsaky, E., Ladenstein, R., Bodenmiller, B., Lion, T., Traver, D., Farlik, M., Schöfer, C., Taschner-Mandl, S., Halbritter, F., Distel, M.
- ID
- ZDB-PUB-240228-8
- Date
- 2024
- Source
- Nature communications 15: 17921792 (Journal)
- Registered Authors
- Distel, Martin, Kirchberger, Stefanie, Traver, David, Wenninger-Weinzierl, Andrea
- Keywords
- none
- Datasets
- GEO:GSE252788
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Bone Marrow/metabolism
- Gene Expression Profiling
- Humans
- Mice
- Neutrophils*
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 38413586 Full text @ Nat. Commun.
Citation
Kirchberger, S., Shoeb, M.R., Lazic, D., Wenninger-Weinzierl, A., Fischer, K., Shaw, L.E., Nogueira, F., Rifatbegovic, F., Bozsaky, E., Ladenstein, R., Bodenmiller, B., Lion, T., Traver, D., Farlik, M., Schöfer, C., Taschner-Mandl, S., Halbritter, F., Distel, M. (2024) Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation. Nature communications. 15:17921792.
Abstract
Neutrophils are evolutionarily conserved innate immune cells playing pivotal roles in host defense. Zebrafish models have contributed substantially to our understanding of neutrophil functions but similarities to human neutrophil maturation have not been systematically characterized, which limits their applicability to studying human disease. Here we show, by generating and analysing transgenic zebrafish strains representing distinct neutrophil differentiation stages, a high-resolution transcriptional profile of neutrophil maturation. We link gene expression at each stage to characteristic transcription factors, including C/ebp-β, which is important for late neutrophil maturation. Cross-species comparison of zebrafish, mouse, and human samples confirms high molecular similarity of immature stages and discriminates zebrafish-specific from pan-species gene signatures. Applying the pan-species neutrophil maturation signature to RNA-sequencing data from human neuroblastoma patients reveals association between metastatic tumor cell infiltration in the bone marrow and an overall increase in mature neutrophils. Our detailed neutrophil maturation atlas thus provides a valuable resource for studying neutrophil function at different stages across species in health and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping