PUBLICATION
Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish
- Authors
- Mahony, C.B., Monteiro, R.
- ID
- ZDB-PUB-240124-6
- Date
- 2024
- Source
- STAR protocols 5: 102810102810 (Journal)
- Registered Authors
- Monteiro, Rui
- Keywords
- Cell isolation, Flow Cytometry, Gene Expression, Model Organisms, Single Cell
- MeSH Terms
-
- Animals
- Bone Marrow*
- Flow Cytometry
- Kidney
- Staining and Labeling
- Zebrafish*
- PubMed
- 38261517 Full text @ STAR Protoc
Citation
Mahony, C.B., Monteiro, R. (2024) Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish. STAR protocols. 5:102810102810.
Abstract
The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marrow cellularity, and analysis of cell populations by flow cytometry. We then detail procedures for May-Grünwald-Giemsa staining for analysis of cellular morphology and phenotyping. For complete details on the use and execution of this protocol, please refer to Mahony et al.1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping