PUBLICATION
Radial astrocyte synchronization modulates the visual system during behavioral-state transitions
- Authors
- Uribe-Arias, A., Rozenblat, R., Vinepinsky, E., Marachlian, E., Kulkarni, A., Zada, D., Privat, M., Topsakalian, D., Charpy, S., Candat, V., Nourin, S., Appelbaum, L., Sumbre, G.
- ID
- ZDB-PUB-231021-54
- Date
- 2023
- Source
- Neuron 111(24): 4040-4057.e6 (Journal)
- Registered Authors
- Appelbaum, Lior, Rozenblat, Rotem, Sumbre, Germán, Zada, David
- Keywords
- behavioral states, functional connectivity, glia, locus coeruleus, neuronal circuits, optic tectum, optogenetics, two-photon calcium imaging, visual system, zebrafish
- MeSH Terms
-
- Animals
- Astrocytes*
- Neurons/physiology
- Norepinephrine
- Superior Colliculi/physiology
- Zebrafish*/physiology
- PubMed
- 37863038 Full text @ Neuron
Citation
Uribe-Arias, A., Rozenblat, R., Vinepinsky, E., Marachlian, E., Kulkarni, A., Zada, D., Privat, M., Topsakalian, D., Charpy, S., Candat, V., Nourin, S., Appelbaum, L., Sumbre, G. (2023) Radial astrocyte synchronization modulates the visual system during behavioral-state transitions. Neuron. 111(24):4040-4057.e6.
Abstract
Glial cells support the function of neurons. Recent evidence shows that astrocytes are also involved in brain computations. To explore whether and how their excitable nature affects brain computations and motor behaviors, we used two-photon Ca2+ imaging of zebrafish larvae expressing GCaMP in both neurons and radial astrocytes (RAs). We found that in the optic tectum, RAs synchronize their Ca2+ transients immediately after the end of an escape behavior. Using optogenetics, ablations, and a genetically encoded norepinephrine sensor, we observed that RA synchronous Ca2+ events are mediated by the locus coeruleus (LC)-norepinephrine circuit. RA synchronization did not induce direct excitation or inhibition of tectal neurons. Nevertheless, it modulated the direction selectivity and the long-distance functional correlations among neurons. This mechanism supports freezing behavior following a switch to an alerted state. These results show that LC-mediated neuro-glial interactions modulate the visual system during transitions between behavioral states.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping