PUBLICATION
Transgenerational effects of BDE-47 to zebrafish based on histomorphometry and toxicogenomic analyses
- Authors
- Huang, W., Shi, X., Zhang, Q., Chen, Y., Zheng, S., Wu, W., Luo, C., Wu, K.
- ID
- ZDB-PUB-231017-55
- Date
- 2023
- Source
- Chemosphere 344: 140401140401 (Journal)
- Registered Authors
- Wu, Kusheng
- Keywords
- BDE-47, Immunotoxicity, Skeleton development, Toxicogenomics, Transgenerational effects, Zebrafish
- MeSH Terms
-
- Animals
- Female
- Halogenated Diphenyl Ethers/analysis
- Larva/genetics
- Male
- Reproduction
- Toxicogenetics
- Water Pollutants, Chemical*/analysis
- Zebrafish*/metabolism
- PubMed
- 37839753 Full text @ Chemosphere
Citation
Huang, W., Shi, X., Zhang, Q., Chen, Y., Zheng, S., Wu, W., Luo, C., Wu, K. (2023) Transgenerational effects of BDE-47 to zebrafish based on histomorphometry and toxicogenomic analyses. Chemosphere. 344:140401140401.
Abstract
Exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) has been found to have an impact on reproductive output and endocrine function in female zebrafish (Danio rerio). However, the transgenerational effects of BDE-47 have not been fully explored in previous reports. In this study, female zebrafish were exposed to BDE-47 for three consecutive weeks. The oogenesis, sex hormones, reproductive histology, and transcriptional profiles of genes along the hypothalamus-pituitary-gonad (HPG) axis were assessed in the exposed-F0 generation. After mating with unexposed males, the transgenerational effects of BDE-47 were evaluated on the basis of histopathology, morphometry and toxicogenome of the unexposed F1 generations at the larval stage. Results indicated that exposure to BDE-47 impaired reproductive capacity, disrupted endocrine system in F0 zebrafish, and compromised craniofacial skeletons and vertebrae development in F1 generations. In addition, through the use of toxicogenomics approach, immune-responsive pathways were found to be significantly enriched, and the transcript expression profiling of immune-related DEGs (IRDs) were dramatically inhibited in F1 generations following maternal BDE-47 exposure, indicating its immunotoxicity to offspring larvae. These findings advance our understanding of the transgenerational toxicity of BDE-47 and advocate for a more comprehensive assessment of other PBDE congeners through histomorphometry and toxicogenomic approaches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping