PUBLICATION

Lack of Stim2 Affects Vision-Dependent Behavior and Sensitivity to Hypoxia

Authors
Wasilewska, I., Majewski, ?., Adamek-Urba?ska, D., Mondal, S.S., Baranykova, S., Gupta, R.K., Bielecki, D., Winata, C.L., Kuznicki, J.
ID
ZDB-PUB-230818-48
Date
2023
Source
Zebrafish   20: 146159146-159 (Journal)
Registered Authors
Gupta, Rishikesh Kumar, Kuznicki, Jacek, Majewski, Lukasz, Wasilewska, Iga, Winata, Cecilia Lanny
Keywords
SOCE, Stim2, behavior, hypoxia, retina, zebrafish
MeSH Terms
  • Larva
  • Mice
  • Stromal Interaction Molecule 2/genetics
  • Homeostasis
  • Animals
  • Hypoxia*
  • Brain
  • Zebrafish*
(all 8)
PubMed
37590563 Full text @ Zebrafish
Abstract
Stromal interaction molecules (STIMs) are endoplasmic reticulum-resident proteins that regulate Ca2+ homeostasis and signaling by store-operated calcium entry (SOCE). The different properties and functions of STIM1 and STIM2 have been described mostly based on work in vitro. STIM2 knockout mice do not survive until adulthood. Therefore, we generated and characterized stim2a and stim2b double-knockout zebrafish. The (stim2a;stim2b)-/- zebrafish did not have any apparent morphological phenotype. However, RNA sequencing revealed 1424 differentially expressed genes. One of the most upregulated genes was annexin A3a, which is a marker of activated microglia. This corresponded well to an increase in Neutral Red staining in the in vivo imaging of the (stim2a;stim2b)-/- zebrafish brain. The lack of Stim2 decreased zebrafish survival under low oxygen conditions. Behavioral tests, such as the visual-motor response test and dark-light preference test, indicated that (stim2a;stim2b)-/- larvae might have problems with vision. This was consistent with the downregulation of many genes that are related to light perception. The periodic acid-Schiff staining of retina sections from adult zebrafish revealed alterations of the stratum pigmentosum, suggesting the involvement of a Stim2-dependent process in visual perception. Altogether, these data reveal new functions for Stim2 in the nervous system.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
waw301
    Insertion
    waw303
      Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available