PUBLICATION
Cadmium exposure promotes ferroptosis by upregulating Heat Shock Protein 70 in vascular endothelial damage of zebrafish
- Authors
- Zhang, T., Yan, W., Liu, C., Duan, W., Duan, Y., Li, Y., Yu, Q., Sun, Y., Tian, J., Zhou, J., Xia, Z., Wang, G., Xu, S.
- ID
- ZDB-PUB-230715-35
- Date
- 2023
- Source
- Ecotoxicology and environmental safety 263: 115241115241 (Journal)
- Registered Authors
- Wang, Guixue
- Keywords
- Cadmium, Ferroptosis, Heat Shock Protein 70, Vascular endothelial cells
- MeSH Terms
-
- Animals
- Cadmium/metabolism
- Endothelial Cells/metabolism
- Ferroptosis*
- HSP70 Heat-Shock Proteins*/genetics
- HSP70 Heat-Shock Proteins*/metabolism
- Zebrafish/metabolism
- PubMed
- 37441943 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhang, T., Yan, W., Liu, C., Duan, W., Duan, Y., Li, Y., Yu, Q., Sun, Y., Tian, J., Zhou, J., Xia, Z., Wang, G., Xu, S. (2023) Cadmium exposure promotes ferroptosis by upregulating Heat Shock Protein 70 in vascular endothelial damage of zebrafish. Ecotoxicology and environmental safety. 263:115241115241.
Abstract
Cadmium (Cd) exposure is a risk factor for endothelial dysfunction and cardiovascular disease. Ferroptosis is a type of cell death that relies on lipid peroxidation. Whether ferroptosis acts in Cd-induced vascular endothelial damage and the underlying mechanisms remain unclear. Herein, we found that Cd resulted in ferroptosis of vascular endothelial cells (ECs) in vivo and in vitro. In the visualized zebrafish embryos, Cd accumulated in vascular ECs, ROS and lipid peroxidation levels were increased, and the oxidoreductase system was disturbed after exposure. Moreover, Cd decreased Gpx4 in ECs and caused smaller mitochondria with increased membrane density. Accompanied by ferroptosis, the number of ECs and the area of the caudal venous plexus in zebrafish embryos were reduced, and the survival rate of HUVECs decreased. These effects were partially reversed by ferrostatin-1 and aggravated by erastin. Mechanistically, an excessive increase in Heat Shock Protein 70 (Hsp70) was identified by transcriptomics after Cd exposure. Inhibition of Hsp70 by VER-155008 or siRNA ameliorated Cd-induced ferroptosis, thereby alleviating endothelial injury. Furthermore, Hsp70 regulated Cd-induced ferroptosis by targeting multiple targets, including Gpx4, Fth1, Nrf2 and Acsl4. Our findings provide a new approach to investigating the endothelial damage of Cd and indicate that regulation of Hsp70 is an important target for alleviating this process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping