PUBLICATION
LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition
- Authors
- Tamaoki, J., Maeda, H., Kobayashi, I., Takeuchi, M., Ohashi, K., Gore, A., Bonkhofer, F., Patient, R., Weinstein, B.M., Kobayashi, M.
- ID
- ZDB-PUB-230625-37
- Date
- 2023
- Source
- Developmental Biology 501: 92-103 (Journal)
- Registered Authors
- Gore, Aniket, Kobayashi, Isao, Kobayashi, Makoto, Maeda, Hiroki, Ohashi, Ken, Patient, Roger K., Takeuchi, Miki, Tamaoki, Junya, Weinstein, Brant M.
- Keywords
- Definitive hematopoiesis, Endothelial-to-hematopoietic transition, Etv2/etsrp, Gfi1/Gfi1b, Hematopoietic stem and progenitor cells, LSD1/KDM1A, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Hematopoiesis/genetics
- Histone Demethylases/genetics
- Mice
- Stem Cells*/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Zebrafish*
- PubMed
- 37353106 Full text @ Dev. Biol.
Citation
Tamaoki, J., Maeda, H., Kobayashi, I., Takeuchi, M., Ohashi, K., Gore, A., Bonkhofer, F., Patient, R., Weinstein, B.M., Kobayashi, M. (2023) LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition. Developmental Biology. 501:92-103.
Abstract
During embryonic development, primitive and definitive waves of hematopoiesis take place to provide proper blood cells for each developmental stage, with the possible involvement of epigenetic factors. We previously found that lysine-specific demethylase 1 (LSD1/KDM1A) promotes primitive hematopoietic differentiation by shutting down the gene expression program of hemangioblasts in an Etv2/Etsrp-dependent manner. In the present study, we demonstrated that zebrafish LSD1 also plays important roles in definitive hematopoiesis in the development of hematopoietic stem and progenitor cells. A combination of genetic approaches and imaging analyses allowed us to show that LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition. Analysis of compound mutant lines with Etv2/Etsrp mutant zebrafish revealed that, unlike in primitive hematopoiesis, this function of LSD1 was independent of Etv2/Etsrp. The phenotype of LSD1 mutant zebrafish during the endothelial-to-hematopoietic transition was similar to that of previously reported compound knockout mice of Gfi1/Gfi1b, which forms a complex with LSD1 and represses endothelial genes. Moreover, co-knockdown of zebrafish Gfi1/Gfi1b genes inhibited the development of HSPCs. We therefore hypothesize that the shutdown of the Gfi1/Gfi1b-target genes during the endothelial-to-hematopoietic transition is one of the key evolutionarily conserved functions of LSD1 in definitive hematopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping