PUBLICATION
hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration
- Authors
- Sun, J., Peterson, E.A., Chen, X., Wang, J.
- ID
- ZDB-PUB-230614-36
- Date
- 2023
- Source
- Nature communications 14: 35053505 (Journal)
- Registered Authors
- Wang, Jinhu
- Keywords
- none
- Datasets
- GEO:GSE216649, GEO:GSE216646, GEO:GSE216647, GEO:GSE216648
- MeSH Terms
-
- Animals
- Coronary Vessels
- Heart
- Hyaluronic Acid*
- Morphogenesis/genetics
- Neovascularization, Pathologic
- Zebrafish*
- PubMed
- 37311876 Full text @ Nat. Commun.
Citation
Sun, J., Peterson, E.A., Chen, X., Wang, J. (2023) hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration. Nature communications. 14:35053505.
Abstract
Although several tissues and chemokines orchestrate coronary formation, the guidance cues for coronary growth remain unclear. Here, we profile the juvenile zebrafish epicardium during coronary vascularization and identify hapln1a+ cells enriched with vascular-regulating genes. hapln1a+ cells not only envelop vessels but also form linear structures ahead of coronary sprouts. Live-imaging demonstrates that coronary growth occurs along these pre-formed structures, with depletion of hapln1a+ cells blocking this growth. hapln1a+ cells also pre-lead coronary sprouts during regeneration and hapln1a+ cell loss inhibits revascularization. Further, we identify serpine1 expression in hapln1a+ cells adjacent to coronary sprouts, and serpine1 inhibition blocks vascularization and revascularization. Moreover, we observe the hapln1a substrate, hyaluronan, forming linear structures along and preceding coronary vessels. Depletion of hapln1a+ cells or serpine1 activity inhibition disrupts hyaluronan structure. Our studies reveal that hapln1a+ cells and serpine1 are required for coronary production by establishing a microenvironment to facilitate guided coronary growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping