PUBLICATION
Betaglycan promoter activity is differentially regulated during myogenesis in zebrafish embryo somites
- Authors
- Ramírez-Vidal, L., Molina-Villa, T., Mendoza, V., Peralta-Álvarez, C.A., Poot-Hernández, A.C., Dotov, D., López-Casillas, F.
- ID
- ZDB-PUB-230525-36
- Date
- 2023
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 252(9): 1162-1179 (Journal)
- Registered Authors
- Keywords
- Tgfbr3 gene promoter, adaxial cells, antero-posterior gradient zebrafish myogenesis, betaglycan, slow twitch somitic muscle
- MeSH Terms
-
- Animals
- Mice
- Muscle Development/physiology
- Muscle Fibers, Slow-Twitch/metabolism
- Proteoglycans/metabolism
- Somites*/metabolism
- Transforming Growth Factor beta/metabolism
- Zebrafish*
- PubMed
- 37222488 Full text @ Dev. Dyn.
Citation
Ramírez-Vidal, L., Molina-Villa, T., Mendoza, V., Peralta-Álvarez, C.A., Poot-Hernández, A.C., Dotov, D., López-Casillas, F. (2023) Betaglycan promoter activity is differentially regulated during myogenesis in zebrafish embryo somites. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(9):1162-1179.
Abstract
Background Betaglycan, also known as the TGFβ type III receptor (Tgfbr3), is a co-receptor that modulates TGFβ family signaling. Tgfbr3 is upregulated during C2C12 myoblast differentiation and expressed in mouse embryos myocytes.
Results To investigate tgfbr3 transcriptional regulation during zebrafish embryonic myogenesis, we cloned a 3.2 kb promoter fragment that drives reporter transcription during C2C12 myoblasts differentiation and in the Tg(tgfbr3:mCherry) transgenic zebrafish. We detect tgfbr3 protein and mCherry expression in the adaxial cells concomitantly with the onset of their radial migration to become slow-twitch muscle fibers in the Tg(tgfbr3:mCherry). Remarkably, this expression displays a measurable antero-posterior somitic gradient expression.
Conclusions tgfbr3 is transcriptionally regulated during somitic muscle development in zebrafish with an antero-posterior gradient expression that preferentially marks the adaxial cells and their descendants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping