PUBLICATION
Smuggling on the Nanoscale-Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo
- Authors
- Hoffmann, M., Gerlach, S., Takamiya, M., Tarazi, S., Hersch, N., Csiszár, A., Springer, R., Dreissen, G., Scharr, H., Rastegar, S., Beil, T., Strähle, U., Merkel, R., Hoffmann, B.
- ID
- ZDB-PUB-230429-66
- Date
- 2023
- Source
- Pharmaceutics 15(4): (Journal)
- Registered Authors
- Beil, Tanja, Rastegar, Sepand, Strähle, Uwe, Takamiya, Masanari
- Keywords
- RNA-transfer, cortical tissue, fusogenic liposomes, lipofection, mammalian cells, zebrafish
- MeSH Terms
- none
- PubMed
- 37111695 Full text @ Pharmaceutics
Citation
Hoffmann, M., Gerlach, S., Takamiya, M., Tarazi, S., Hersch, N., Csiszár, A., Springer, R., Dreissen, G., Scharr, H., Rastegar, S., Beil, T., Strähle, U., Merkel, R., Hoffmann, B. (2023) Smuggling on the Nanoscale-Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo. Pharmaceutics. 15(4):.
Abstract
The efficient and biocompatible transfer of nucleic acids into mammalian cells for research applications or medical purposes is a long-standing, challenging task. Viral transduction is the most efficient transfer system, but often entails high safety levels for research and potential health impairments for patients in medical applications. Lipo- or polyplexes are commonly used transfer systems but result in comparably low transfer efficiencies. Moreover, inflammatory responses caused by cytotoxic side effects were reported for these transfer methods. Often accountable for these effects are various recognition mechanisms for transferred nucleic acids. Using commercially available fusogenic liposomes (Fuse-It-mRNA), we established highly efficient and fully biocompatible transfer of RNA molecules for in vitro as well as in vivo applications. We demonstrated bypassing of endosomal uptake routes and, therefore, of pattern recognition receptors that recognize nucleic acids with high efficiency. This may underlie the observed almost complete abolishment of inflammatory cytokine responses. RNA transfer experiments into zebrafish embryos and adult animals fully confirmed the functional mechanism and the wide range of applications from single cells to organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping