ZFIN ID: ZDB-PERS-010827-6
Rastegar, Sepand
Email: sepand.rastegar@kit.edu
URL: http://bip.ibcs.kit.edu/rastegar.php
Affiliation: Rastegar Lab
Address: Institute of Biological and Chemical Systems - Biological Information Processing Karlsruhe Institute of Technology Postfach 3640 Karlsruhe, D-76021
Country: Germany
Phone: +49-721-608-22886
Fax: +49-721-608-23354
ORCID ID: 0000-0003-4411-5646

I have been employed at the Institute of Biological and Chemical Systems - Biological Information Processing (formerly known as ITG) since January 2005, initially as a project leader and, since 2020, as an independent group leader. Prior to joining the Karlsruhe Institute of Technology, I held the following key roles:

INSERM Fellow (2002-present).
Postdoctoral Researcher at IGBMC, France (2000-2002).
Obtained a Ph.D. degree from the University of Ulm, Germany (1997-2000).
Earned a Diploma from the University of Strasbourg, France (1995-1997).

Research interests:
My research focuses on comprehensively characterizing the molecular mechanisms governing both zebrafish embryonic and adult constitutive and regenerative neurogenesis, with a particular emphasis on transcriptional regulation during these processes.

Seliwjorstow, A., Takamiya, M., Rastegar, S., Pianowski, Z. (2024) Reversible Influence of Hemipiperazine Photochromism on the Early Development of Zebrafish Embryo. Chembiochem : a European journal of chemical biology. 25(8):e202400143
Štefl, M., Takamiya, M., Middel, V., Tekpınar, M., Nienhaus, K., Beil, T., Rastegar, S., Strähle, U., Nienhaus, G.U. (2024) Caveolae disassemble upon membrane lesioning and foster cell survival. iScience. 27:108849108849
Pellegrini, E., Fernezelian, D., Malleret, C., Gueguen, M.M., Patche-Firmin, J., Rastegar, S., Meilhac, O., Diotel, N. (2023) Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood-brain barrier?. The Journal of comparative neurology. 531(17):1828-1845
Gence, L., Fernezelian, D., Meilhac, O., Rastegar, S., Bascands, J.L., Diotel, N. (2023) Insulin signaling promotes neurogenesis in the brain of adult zebrafish. The Journal of comparative neurology. 531(17):1812-1827
Chen, F., Köhler, M., Cucun, G., Takamiya, M., Kizil, C., Cosacak, M.I., Rastegar, S. (2023) sox1a:eGFP transgenic line and single-cell transcriptomics reveal the origin of zebrafish intraspinal serotonergic neurons. iScience. 26:107342107342
Hoffmann, M., Gerlach, S., Takamiya, M., Tarazi, S., Hersch, N., Csiszár, A., Springer, R., Dreissen, G., Scharr, H., Rastegar, S., Beil, T., Strähle, U., Merkel, R., Hoffmann, B. (2023) Smuggling on the Nanoscale-Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo. Pharmaceutics. 15(4):
Mhalhel, K., Sicari, M., Pansera, L., Chen, J., Levanti, M., Diotel, N., Rastegar, S., Germanà, A., Montalbano, G. (2023) Zebrafish: A Model Deciphering the Impact of Flavonoids on Neurodegenerative Disorders. Cells. 12(2):
Narra, S.S., Rondeau, P., Fernezelian, D., Gence, L., Ghaddar, B., Bourdon, E., Lefebvre d'Hellencourt, C., Rastegar, S., Diotel, N. (2022) Distribution of microglia/immune cells in the brain of adult zebrafish in homeostatic and regenerative conditions: Focus on oxidative stress during brain repair. The Journal of comparative neurology. 531(2):238-255
Lübke, L., Zhang, G., Strähle, U., Rastegar, S. (2022) mdka Expression Is Associated with Quiescent Neural Stem Cells during Constitutive and Reactive Neurogenesis in the Adult Zebrafish Telencephalon. Brain sciences. 12(2):
Khan, A., Molitor, A., Mayeur, S., Zhang, G., Rinaldi, B., Lannes, B., Lhermitte, B., Umair, M., Arold, S.T., Friant, S., Rastegar, S., Anheim, M., Bahram, S., Carapito, R. (2021) A Homozygous Missense Variant in PPP1R1B/DARPP-32 Is Associated With Generalized Complex Dystonia. Movement disorders : official journal of the Movement Disorder Society. 37(2):365-374
Zhang, G., Lübke, L., Chen, F., Beil, T., Takamiya, M., Diotel, N., Strähle, U., Rastegar, S. (2021) Neuron-Radial Glial Cell Communication via BMP/Id1 Signaling Is Key to Long-Term Maintenance of the Regenerative Capacity of the Adult Zebrafish Telencephalon. Cells. 10(10):
Gourain, V., Armant, O., Lübke, L., Diotel, N., Rastegar, S., Strähle, U. (2021) Multi-Dimensional Transcriptome Analysis Reveals Modulation of Cholesterol Metabolism as Highly Integrated Response to Brain Injury. Frontiers in neuroscience. 15:671249
Sulliman, N.C., Ghaddar, B., Gence, L., Patche, J., Rastegar, S., Meilhac, O., Diotel, N. (2021) HDL biodistribution and brain receptors in zebrafish, using HDLs as vectors for targeting endothelial cells and neural progenitors. Scientific Reports. 11:6439
Ghaddar, B., Lübke, L., Couret, D., Rastegar, S., Diotel, N. (2021) Cellular Mechanisms Participating in Brain Repair of Adult Zebrafish and Mammals after Injury. Cells. 10(2):
Diotel, N., Lübke, L., Strähle, U., Rastegar, S. (2020) Common and Distinct Features of Adult Neurogenesis and Regeneration in the Telencephalon of Zebrafish and Mammals. Frontiers in neuroscience. 14:568930
Takamiya, M., Stegmaier, J., Kobitski, A.Y., Schott, B., Weger, B.D., Margariti, D., Cereceda Delgado, A.R., Gourain, V., Scherr, T., Yang, L., Sorge, S., Otte, J.C., Hartmann, V., van Wezel, J., Stotzka, R., Reinhard, T., Schlunck, G., Dickmeis, T., Rastegar, S., Mikut, R., Nienhaus, G.U., Strähle, U. (2020) Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves. PLoS Genetics. 16:e1008774
Zhang, G., Ferg, M., Lübke, L., Takamiya, M., Beil, T., Gourain, V., Diotel, N., Strähle, U., Rastegar, S. (2020) Bone morphogenetic protein signaling regulates Id1-mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module. Stem cells (Dayton, Ohio). 38(7):875-889
Rastegar, S., Parimisetty, A., Cassam-Sulliman, N., Narra Sai, S., Weber, S., Rastegar, M., Viranaicken, W., Couret, D., Planesse, C., Strähle, U., Meilhac, O., Lefebvre d'Hellencourt, C., Diotel, N. (2019) Expression of adiponectin receptors in the brain of adult zebrafish and mouse: Links with neurogenic niches and brain repair. The Journal of comparative neurology. 527(14):2317-2333
Gerber, V., Yang, L., Takamiya, M., Ribes, V., Gourain, V., Peravali, R., Stegmaier, J., Mikut, R., Reischl, M., Ferg, M., Rastegar, S., Strähle, U. (2019) The HMG box transcription factors Sox1a and b specify a new class of glycinergic interneurons in the spinal cord of zebrafish embryos. Development (Cambridge, England). 146(4):
Weger, M., Diotel, N., Weger, B.D., Beil, T., Zaucker, A., Eachus, H.L., Oakes, J.A., do Rego, J.L., Storbeck, K.H., Gut, P., Straehle, U., Rastegar, S., Mueller, F., Krone, N. (2018) Expression and activity profiling of the steroidogenic enzymes of glucocorticoid biosynthesis and the fdx1 co-factors in zebrafish. Journal of neuroendocrinology. 30(4):e12586
Angelin, A., Kassel, O., Rastegar, S., Strähle, U., Niemeyer, C.M. (2016) Protein-Functionalized DNA Nanostructures as Tools to Control Transcription in Zebrafish Embryos. ChemistryOpen. 6:33-39
Middel, V., Zhou, L., Takamiya, M., Beil, T., Shahid, M., Roostalu, U., Grabher, C., Rastegar, S., Reischl, M., Nienhaus, G.U., Strähle, U. (2016) Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair. Nature communications. 7:12875
Strähle, U., Ferg, M., Armant, O., Gradl, M., Kaufmann, L., Rastegar, S. (2016) HeRBi: Helmholtz Repository of Bioparts. Zebrafish. 13(3):234-5
Takamiya, M., Xu, F., Suhonen, H., Gourain, V., Yang, L., Ho, N.Y., Helfen, L., Schröck, A., Etard, C., Grabher, C., Rastegar, S., Schlunck, G., Reinhard, T., Baumbach, T., Strähle, U. (2016) Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development. Scientific Reports. 6:25046
Shahid, M., Takamiya, M., Stegmaier, J., Middel, V., Gradl, M., Klüver, N., Mikut, R., Dickmeis, T., Scholz, S., Rastegar, S., Yang, L., Strähle, U. (2016) Zebrafish biosensor for toxicant induced muscle hyperactivity. Scientific Reports. 6:23768
Rastegar, S., Strähle, U. (2016) The Zebrafish as Model for Deciphering the Regulatory Architecture of Vertebrate Genomes. Advances in genetics. 95:195-216
Diotel, N., Beil, T., Strähle, U., Rastegar, S. (2015) Differential expression of id genes and their potential regulator znf238 in zebrafish adult neural progenitor cells and neurons suggests distinct functions in adult neurogenesis. Gene expression patterns : GEP. 19(1-2):1-13
Diotel, N., Viales, R.R., Armant, O., März, M., Ferg, M., Rastegar, S., Strähle, U. (2015) Comprehensive expression map of transcription regulators in the adult zebrafish telencephalon reveals distinct neurogenic niches. The Journal of comparative neurology. 523(8):1202-21
Naville, M., Ishibashi, M., Ferg, M., Bengani, H., Rinkwitz, S., Krecsmarik, M., Hawkins, T.A., Wilson, S.W., Manning, E., Chilamakuri, C.S., Wilson, D.I., Louis, A., Lucy Raymond, F., Rastegar, S., Strähle, U., Lenhard, B., Bally-Cuif, L., van Heyningen, V., FitzPatrick, D.R., Becker, T.S., Roest Crollius, H. (2015) Long-range evolutionary constraints reveal cis-regulatory interactions on the human X chromosome. Nature communications. 6:6904
Rodriguez Viales, R., Diotel, N., Ferg, M., Armant, O., Eich, J., Alunni, A., März, M., Bally-Cuif, L., Rastegar, S., Strähle, U. (2015) The helix-loop-helix protein Id1 controls stem cell proliferation during regenerative neurogenesis in the adult zebrafish telencephalon. Stem cells (Dayton, Ohio). 33(3):892-903
Kobitski, A.Y., Otte, J.C., Takamiya, M., Schäfer, B., Mertes, J., Stegmaier, J., Rastegar, S., Rindone, F., Hartmann, V., Stotzka, R., García, A., van Wezel, J., Mikut, R., Strähle, U., Nienhaus, G.U. (2015) An ensemble-averaged, cell density-based digital model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution. Scientific Reports. 5:8601
Takamiya, M., Weger, B.D., Schindler, S., Beil, T., Yang, L., Armant, O., Ferg, M., Schlunck, G., Reinhard, T., Dickmeis, T., Rastegar, S., Strähle, U. (2015) Molecular Description of Eye Defects in the Zebrafish Pax6b Mutant, sunrise, Reveals a Pax6b-Dependent Genetic Network in the Developing Anterior Chamber. PLoS One. 10:e0117645
Schmidt, R., Beil, T., Strähle, U., Rastegar, S. (2014) Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration. Journal of visualized experiments : JoVE. (90):e51753
Stegmaier, J., Shahid, M., Takamiya, M., Yang, L., Rastegar, S., Reischl, M., Strähle, U., and Mikut, R. (2014) Automated prior knowledge-based quantification of neuronal patterns in the spinal cord of zebrafish. Bioinformatics (Oxford, England). 30(5):726-33
Ferg, M., Armant, O., Yang, L., Dickmeis, T., Rastegar, S., and Strähle, U. (2014) Gene transcription in the zebrafish embryo: regulators and networks. Briefings in Functional Genomics. 13(2):131-43
Nepal, C., Hadzhiev, Y., Previty, C., Haberle, V., Li, N., Takahashi, H., Suzuki, A.M., Sheng, Y., Abdelhamid, R., Anand, S., Gehrig, J., Akalin, A., Kockx, C., van der Sloot, A., van Ijcken, W., Armant, O., Rastegar, S., Straehle, U., Stupka, E., Carninci, P., Lenhard, B., and Muller, F. (2013) Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis. Genome research. 23(11):1938-50
Armant, O., März, M., Schmidt, R., Ferg, M., Diotel, N., Ertzer, R., Bryne, J.C., Yang, L., Baader, I., Reischl, M., Legradi, J., Mikut, R., Stemple, D., Ijcken, W.V., van der Sloot, A., Lenhard, B., Strähle, U., and Rastegar, S. (2013) Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos. Developmental Biology. 380(2):351-62
Weger, M., Weger, B.D., Diotel, N., Rastegar, S., Hirota, T., Kay, S.A., Strähle, U., and Dickmeis, T. (2013) Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock. Developmental Biology. 380(2):259-73
Ho, N.Y., Yang, L., Legradi, J., Armant, O., Takamiya, M., Rastegar, S., and Strähle, U. (2013) Gene Responses in the Central Nervous System of Zebrafish Embryos Exposed to the Neurotoxicant Methyl Mercury. Environmental science & technology. 47(7):3316-25
Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H., and Braunbeck, T. (2012) Zebrafish embryos as an alternative to animal experiments-A commentary on the definition of the onset of protected life stages in animal welfare regulations. Reproductive toxicology (Elmsford, N.Y.). 33(2):128-132
März, M., Schmidt, R., Rastegar, S., and Strähle, U. (2011) Regenerative response following stab injury in the adult zebrafish telencephalon. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(9):2221-2231
März, M., Schmidt, R., Rastegar, S., and Strähle, U. (2010) Expression of the transcription factor Olig2 in proliferating cells in the adult zebrafish telencephalon. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(12):3336-3349
Yang, L., Rastegar, S., and Strähle, U. (2010) Regulatory interactions specifying Kolmer-Agduhr interneurons. Development (Cambridge, England). 137(16):2713-2722
Rastegar, S., Hess, I., Dickmeis, T., Nicod, J.C., Ertzer, R., Hadzhiev, Y., Thies, W.G., Scherer, G., and Strähle, U. (2008) The words of the regulatory code are arranged in a variable manner in highly conserved enhancers. Developmental Biology. 318(2):366-377
Strähle, U., and Rastegar, S. (2008) Conserved non-coding sequences and transcriptional regulation. Brain research bulletin. 75(2-4):225-230
Vinothkumar, S., Rastegar, S., Takamiya, M., Ertzer, R., and Strähle, U. (2008) Sequential and cooperative action of Fgfs and Shh in the zebrafish retina. Developmental Biology. 314(1):200-214
Ertzer, R., Muller, F., Hadzhiev, Y., Rathnam, S., Fischer, N., Rastegar, S., and Strähle, U. (2007) Cooperation of sonic hedgehog enhancers in midline expression. Developmental Biology. 301(2):578-589
Lam, C.S., Rastegar, S., and Strähle, U. (2006) Distribution of cannabinoid receptor 1 in the CNS of zebrafish. Neuroscience. 138(1):83-95
Norton, W.H., Mangoli, M., Lele, Z., Pogoda, H.M., Diamond, B., Mercurio, S., Russell, C., Teraoka, H., Stickney, H.L., Rauch, G.J., Heisenberg, C.P., Houart, C., Schilling, T.F., Frohnhoefer, H.G., Rastegar, S., Neumann, C.J., Gardiner, R.M., Strähle, U., Geisler, R., Rees, M., Talbot, W.S., and Wilson, S.W. (2005) Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphe neurones and cranial motoneurones. Development (Cambridge, England). 132(4):645-658
Blader, P., Lam, C.S., Rastegar, S., Scardigli, R., Nicod, J.C., Simplicio, N., Plessy, C., Fischer, N., Schuurmans, C., Guillemot, F., and Strähle, U. (2004) Conserved and acquired features of neurogenin1 regulation. Development (Cambridge, England). 131(22):5627-5637
Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L. (2004) Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary. Development (Cambridge, England). 131(9):1993-2006
Strähle, U., Lam, C.S., Ertzer, R., and Rastegar, S. (2004) Vertebrate floor-plate specification: variations on common themes. Trends in genetics : TIG. 20(3):155-162
Dickmeis, T., Plessy, C., Rastegar, S., Aanstad, P., Herwig, R., Chalmel, F., Fischer, N., and Strähle, U. (2004) Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo. Genome research. 14(2):228-238
Dufourcq, P., Rastegar, S., Strähle, U., and Blader, P. (2004) Parapineal specific expression of gfi1 in the zebrafish epithalamus. Gene expression patterns : GEP. 4(1):53-57
Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U. (2002) A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2. Developmental Biology. 252(1):1-14
Dickmeis, T., Rastegar, S., Lam, C.S., Aanstad, P., Clark, M., Fischer, N., Rosa, F., Korzh, V., and Strähle, U. (2002) Expression of the helix-loop-helix gene id3 in the zebrafish embryo. Mechanisms of Development. 113(1):99-109
Dickmeis, T., Rastegar, S., Aanstad, P., Clark, M., Fischer, N., Plessy, C., Rosa, F., Korzh, V., Strähle, U. (2001) Expression of brain subtype creatine kinase in the zebrafish embryo. Mechanisms of Development. 109(2):409-412
Dickmeis, T., Rastegar, S., Aanstad, P., Clark, M., Fischer, N., Korzh, V., and Strähle, U. (2001) Expression of the anti-dorsalizing morphogenetic protein gene in the zebrafish embryo. Development genes and evolution. 211(11):568-572
Müller, F., Blader, P., Rastegar, S., Fischer, N., Knochel, W., Strähle, U. (1999) Characterization of zebrafish smad1, smad2 and smad5: the amino-terminus of Smad1 and Smad5 is required for specific function in the embryo. Mechanisms of Development. 88(1):73-88
Blader, P., Rastegar, S., Fischer, N., and Strähle, U. (1997) Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science (New York, N.Y.). 278(5345):1937-1940

Friedle, H., Rastegar, S., Paul, H., Kaufmann, E. and Knochel, W. (1998) Xvent-1 mediates BMP-4-induced suppression of the dorsal-lip-specific early response gene XFD-1' in Xenopus embryos. EMBO J. 17(8):2298-2307.

Rastegar, S., Friedle, H., Frommer, G. and Knochel, W. (1999) Transcriptional regulation of Xvent homeobox genes. Mech. Dev. 81:139-149.

Hennigfeld, K.A., Rastegar, S., Adler, G. and Knochel, W. (2000) Smadl and Smad4 are components of the BMP-4 induced transcription complex of the Xvent-2B promoter. J. Biol. Chem. 275(29):21827-21835.

Hennigfeld, K.A., Friedle, H., Rastegar, S. and Knochel, W. (2002) Autoregulation of Xvent-2B: Direct interaction and functional cooperation of Xvent-2 and Smadl. J. Biol. Chem. 277:2097-2103.