ZFIN ID: ZDB-PERS-010827-6
Rastegar, Sepand
Email: sepand.rastegar@kit.edu
URL: http://www.itg.kit.edu/straehle.php
Affiliation: Strähle Lab
Address: Institute of Toxicology and Genetics Karlsruhe Institute of Technology Postfach 3640 Karlsruhe, D-76021 Germany
Country:
Phone: +49-721-608-22886
Fax: +49-721-608-23354
Orcid ID: 0000-0003-4411-5646


BIOGRAPHY AND RESEARCH INTERESTS
Biography:
Since January 2005 working at the Institute of Toxicology and Genetics, Germany.
2002-2005: INSERM fellow, IGBMC, France.
2000-2002: Postdoctoral employment, IGBMC, France.
1997-2000: Ph.D. degree from the University of Ulm, Germany.
1995-1997: Diploma from the University of Strasbourg, France.

Research interests:
Elucidation and functional characterization of the regulatory elements that control midline specific genes.
Characterization of molecular mechanisms that regulate zebrafish embryonic and adult neurogenesis.


PUBLICATIONS
Angelin, A., Kassel, O., Rastegar, S., Strähle, U., Niemeyer, C.M. (2016) Protein-Functionalized DNA Nanostructures as Tools to Control Transcription in Zebrafish Embryos. ChemistryOpen. 6:33-39
Middel, V., Zhou, L., Takamiya, M., Beil, T., Shahid, M., Roostalu, U., Grabher, C., Rastegar, S., Reischl, M., Nienhaus, G.U., Strähle, U. (2016) Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair. Nature communications. 7:12875
Strähle, U., Ferg, M., Armant, O., Gradl, M., Kaufmann, L., Rastegar, S. (2016) HeRBi: Helmholtz Repository of Bioparts. Zebrafish. 13(3):234-5
Takamiya, M., Xu, F., Suhonen, H., Gourain, V., Yang, L., Ho, N.Y., Helfen, L., Schröck, A., Etard, C., Grabher, C., Rastegar, S., Schlunck, G., Reinhard, T., Baumbach, T., Strähle, U. (2016) Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development. Scientific Reports. 6:25046
Shahid, M., Takamiya, M., Stegmaier, J., Middel, V., Gradl, M., Klüver, N., Mikut, R., Dickmeis, T., Scholz, S., Rastegar, S., Yang, L., Strähle, U. (2016) Zebrafish biosensor for toxicant induced muscle hyperactivity. Scientific Reports. 6:23768
Rastegar, S., Strähle, U. (2016) The Zebrafish as Model for Deciphering the Regulatory Architecture of Vertebrate Genomes. Advances in genetics. 95:195-216
Diotel, N., Beil, T., Strähle, U., Rastegar, S. (2015) Differential expression of id genes and their potential regulator znf238 in zebrafish adult neural progenitor cells and neurons suggests distinct functions in adult neurogenesis. Gene Expression Patterns. 19(1-2):1-13
Diotel, N., Viales, R.R., Armant, O., März, M., Ferg, M., Rastegar, S., Strähle, U. (2015) Comprehensive expression map of transcription regulators in the adult zebrafish telencephalon reveals distinct neurogenic niches. The Journal of comparative neurology. 523(8):1202-21
Naville, M., Ishibashi, M., Ferg, M., Bengani, H., Rinkwitz, S., Krecsmarik, M., Hawkins, T.A., Wilson, S.W., Manning, E., Chilamakuri, C.S., Wilson, D.I., Louis, A., Lucy Raymond, F., Rastegar, S., Strähle, U., Lenhard, B., Bally-Cuif, L., van Heyningen, V., FitzPatrick, D.R., Becker, T.S., Roest Crollius, H. (2015) Long-range evolutionary constraints reveal cis-regulatory interactions on the human X chromosome. Nature communications. 6:6904
Rodriguez Viales, R., Diotel, N., Ferg, M., Armant, O., Eich, J., Alunni, A., März, M., Bally-Cuif, L., Rastegar, S., Strähle, U. (2015) The helix-loop-helix protein Id1 controls stem cell proliferation during regenerative neurogenesis in the adult zebrafish telencephalon. Stem Cells. 33(3):892-903
Kobitski, A.Y., Otte, J.C., Takamiya, M., Schäfer, B., Mertes, J., Stegmaier, J., Rastegar, S., Rindone, F., Hartmann, V., Stotzka, R., García, A., van Wezel, J., Mikut, R., Strähle, U., Nienhaus, G.U. (2015) An ensemble-averaged, cell density-based digital model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution. Scientific Reports. 5:8601
Takamiya, M., Weger, B.D., Schindler, S., Beil, T., Yang, L., Armant, O., Ferg, M., Schlunck, G., Reinhard, T., Dickmeis, T., Rastegar, S., Strähle, U. (2015) Molecular Description of Eye Defects in the Zebrafish Pax6b Mutant, sunrise, Reveals a Pax6b-Dependent Genetic Network in the Developing Anterior Chamber. PLoS One. 10:e0117645
Schmidt, R., Beil, T., Strähle, U., Rastegar, S. (2014) Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration. Journal of visualized experiments : JoVE. (90):e51753
Stegmaier, J., Shahid, M., Takamiya, M., Yang, L., Rastegar, S., Reischl, M., Strähle, U., and Mikut, R. (2014) Automated prior knowledge-based quantification of neuronal patterns in the spinal cord of zebrafish. Bioinformatics (Oxford, England). 30(5):726-33
Ferg, M., Armant, O., Yang, L., Dickmeis, T., Rastegar, S., and Strähle, U. (2014) Gene transcription in the zebrafish embryo: regulators and networks. Briefings in Functional Genomics. 13(2):131-43
Nepal, C., Hadzhiev, Y., Previty, C., Haberle, V., Li, N., Takahashi, H., Suzuki, A.M., Sheng, Y., Abdelhamid, R., Anand, S., Gehrig, J., Akalin, A., Kockx, C., van der Sloot, A., van Ijcken, W., Armant, O., Rastegar, S., Straehle, U., Stupka, E., Carninci, P., Lenhard, B., and Muller, F. (2013) Dynamic regulation of the transcription initiation landscape at single nucleotide resolution during vertebrate embryogenesis. Genome research. 23(11):1938-50
Armant, O., März, M., Schmidt, R., Ferg, M., Diotel, N., Ertzer, R., Bryne, J.C., Yang, L., Baader, I., Reischl, M., Legradi, J., Mikut, R., Stemple, D., Ijcken, W.V., van der Sloot, A., Lenhard, B., Strähle, U., and Rastegar, S. (2013) Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos. Developmental Biology. 380(2):351-62
Weger, M., Weger, B.D., Diotel, N., Rastegar, S., Hirota, T., Kay, S.A., Strähle, U., and Dickmeis, T. (2013) Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock. Developmental Biology. 380(2):259-73
Ho, N.Y., Yang, L., Legradi, J., Armant, O., Takamiya, M., Rastegar, S., and Strähle, U. (2013) Gene Responses in the Central Nervous System of Zebrafish Embryos Exposed to the Neurotoxicant Methyl Mercury. Environ. Sci. Technol.. 47(7):3316-25
Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H., and Braunbeck, T. (2012) Zebrafish embryos as an alternative to animal experiments-A commentary on the definition of the onset of protected life stages in animal welfare regulations. Reproductive toxicology (Elmsford, N.Y.). 33(2):128-132
März, M., Schmidt, R., Rastegar, S., and Strähle, U. (2011) Regenerative response following stab injury in the adult zebrafish telencephalon. Developmental dynamics : an official publication of the American Association of Anatomists. 240(9):2221-2231
März, M., Schmidt, R., Rastegar, S., and Strähle, U. (2010) Expression of the transcription factor Olig2 in proliferating cells in the adult zebrafish telencephalon. Developmental dynamics : an official publication of the American Association of Anatomists. 239(12):3336-3349
Yang, L., Rastegar, S., and Strähle, U. (2010) Regulatory interactions specifying Kolmer-Agduhr interneurons. Development (Cambridge, England). 137(16):2713-2722
Rastegar, S., Hess, I., Dickmeis, T., Nicod, J.C., Ertzer, R., Hadzhiev, Y., Thies, W.G., Scherer, G., and Strähle, U. (2008) The words of the regulatory code are arranged in a variable manner in highly conserved enhancers. Developmental Biology. 318(2):366-377
Strähle, U., and Rastegar, S. (2008) Conserved non-coding sequences and transcriptional regulation. Brain research bulletin. 75(2-4):225-230
Vinothkumar, S., Rastegar, S., Takamiya, M., Ertzer, R., and Strähle, U. (2008) Sequential and cooperative action of Fgfs and Shh in the zebrafish retina. Developmental Biology. 314(1):200-214
Ertzer, R., Muller, F., Hadzhiev, Y., Rathnam, S., Fischer, N., Rastegar, S., and Strähle, U. (2007) Cooperation of sonic hedgehog enhancers in midline expression. Developmental Biology. 301(2):578-589
Lam, C.S., Rastegar, S., and Strähle, U. (2006) Distribution of cannabinoid receptor 1 in the CNS of zebrafish. Neuroscience. 138(1):83-95
Norton, W.H., Mangoli, M., Lele, Z., Pogoda, H.M., Diamond, B., Mercurio, S., Russell, C., Teraoka, H., Stickney, H.L., Rauch, G.J., Heisenberg, C.P., Houart, C., Schilling, T.F., Frohnhoefer, H.G., Rastegar, S., Neumann, C.J., Gardiner, R.M., Strähle, U., Geisler, R., Rees, M., Talbot, W.S., and Wilson, S.W. (2005) Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphe neurones and cranial motoneurones. Development (Cambridge, England). 132(4):645-658
Blader, P., Lam, C.S., Rastegar, S., Scardigli, R., Nicod, J.C., Simplicio, N., Plessy, C., Fischer, N., Schuurmans, C., Guillemot, F., and Strähle, U. (2004) Conserved and acquired features of neurogenin1 regulation. Development (Cambridge, England). 131(22):5627-5637
Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L. (2004) Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary. Development (Cambridge, England). 131(9):1993-2006
Strähle, U., Lam, C.S., Ertzer, R., and Rastegar, S. (2004) Vertebrate floor-plate specification: variations on common themes. Trends in genetics : TIG. 20(3):155-162
Dickmeis, T., Plessy, C., Rastegar, S., Aanstad, P., Herwig, R., Chalmel, F., Fischer, N., and Strähle, U. (2004) Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo. Genome research. 14(2):228-238
Dufourcq, P., Rastegar, S., Strähle, U., and Blader, P. (2004) Parapineal specific expression of gfi1 in the zebrafish epithalamus. Gene Expression Patterns. 4(1):53-57
Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U. (2002) A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2. Developmental Biology. 252(1):1-14
Dickmeis, T., Rastegar, S., Lam, C.S., Aanstad, P., Clark, M., Fischer, N., Rosa, F., Korzh, V., and Strähle, U. (2002) Expression of the helix-loop-helix gene id3 in the zebrafish embryo. Mechanisms of Development. 113(1):99-109
Dickmeis, T., Rastegar, S., Aanstad, P., Clark, M., Fischer, N., Korzh, V., and Strähle, U. (2001) Expression of the anti-dorsalizing morphogenetic protein gene in the zebrafish embryo. Development genes and evolution. 211(11):568-572
Dickmeis, T., Rastegar, S., Aanstad, P., Clark, M., Fischer, N., Plessy, C., Rosa, F., Korzh, V., Strähle, U. (2001) Expression of brain subtype creatine kinase in the zebrafish embryo. Mechanisms of Development. 109(2):409-412
Müller, F., Blader, P., Rastegar, S., Fischer, N., Knochel, W., Strähle, U. (1999) Characterization of zebrafish smad1, smad2 and smad5: the amino-terminus of Smad1 and Smad5 is required for specific function in the embryo. Mechanisms of Development. 88(1):73-88
Blader, P., Rastegar, S., Fischer, N., and Strähle, U. (1997) Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science (New York, N.Y.). 278(5345):1937-1940

NON-ZEBRAFISH PUBLICATIONS
Friedle, H., Rastegar, S., Paul, H., Kaufmann, E. and Knochel, W. (1998) Xvent-1 mediates BMP-4-induced suppression of the dorsal-lip-specific early response gene XFD-1' in Xenopus embryos. EMBO J. 17(8):2298-2307.

Rastegar, S., Friedle, H., Frommer, G. and Knochel, W. (1999) Transcriptional regulation of Xvent homeobox genes. Mech. Dev. 81:139-149.

Hennigfeld, K.A., Rastegar, S., Adler, G. and Knochel, W. (2000) Smadl and Smad4 are components of the BMP-4 induced transcription complex of the Xvent-2B promoter. J. Biol. Chem. 275(29):21827-21835.

Hennigfeld, K.A., Friedle, H., Rastegar, S. and Knochel, W. (2002) Autoregulation of Xvent-2B: Direct interaction and functional cooperation of Xvent-2 and Smadl. J. Biol. Chem. 277:2097-2103.