PUBLICATION
Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice
- Authors
- Ahmad-Mansour, N., Elhawy, M.I., Huc-Brandt, S., Youssouf, N., Pätzold, L., Martin, M., Abdel-Wadood, N., Aljohmani, A., Morsli, M., Krasteva-Christ, G., Becker, S.L., Yildiz, D., Lavigne, J.P., Gannoun-Zaki, L., Bischoff, M., Molle, V.
- ID
- ZDB-PUB-221127-9
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(22): (Journal)
- Registered Authors
- Keywords
- SapS, Staphylococcus aureus, acid phosphatase, biofilm, inflammatory response, macrophage survival, oxidative stress, virulence
- MeSH Terms
-
- Acid Phosphatase
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Mice
- Staphylococcal Infections*/microbiology
- Staphylococcus aureus*
- Virulence
- Virulence Factors/genetics
- Virulence Factors/metabolism
- Zebrafish/metabolism
- PubMed
- 36430506 Full text @ Int. J. Mol. Sci.
Citation
Ahmad-Mansour, N., Elhawy, M.I., Huc-Brandt, S., Youssouf, N., Pätzold, L., Martin, M., Abdel-Wadood, N., Aljohmani, A., Morsli, M., Krasteva-Christ, G., Becker, S.L., Yildiz, D., Lavigne, J.P., Gannoun-Zaki, L., Bischoff, M., Molle, V. (2022) Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice. International Journal of Molecular Sciences. 23(22):.
Abstract
Staphylococcus aureus possesses a large arsenal of immune-modulating factors, enabling it to bypass the immune system's response. Here, we demonstrate that the acid phosphatase SapS is secreted during macrophage infection and promotes its intracellular survival in this type of immune cell. In animal models, the SA564 sapS mutant demonstrated a significantly lower bacterial burden in liver and renal tissues of mice at four days post infection in comparison to the wild type, along with lower pathogenicity in a zebrafish infection model. The SA564 sapS mutant elicits a lower inflammatory response in mice than the wild-type strain, while S. aureus cells harbouring a functional sapS induce a chemokine response that favours the recruitment of neutrophils to the infection site. Our in vitro and quantitative transcript analysis show that SapS has an effect on S. aureus capacity to adapt to oxidative stress during growth. SapS is also involved in S. aureus biofilm formation. Thus, this study shows for the first time that SapS plays a significant role during infection, most likely through inhibiting a variety of the host's defence mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping