PUBLICATION
Optogenetic Protein Cleavage in Zebrafish Embryos
- Authors
- Brown, W., Albright, S., Tsang, M., Deiters, A.
- ID
- ZDB-PUB-221006-1
- Date
- 2022
- Source
- Chembiochem : a European journal of chemical biology 23(23): e202200297 (Journal)
- Registered Authors
- Tsang, Michael
- Keywords
- Optogenetics * Zebrafish * Photocleavable protein * PhoCl * Apoptosis
- MeSH Terms
-
- Animals
- Apoptosis
- Optogenetics*
- Protein Transport
- Proteolysis
- Zebrafish*/genetics
- PubMed
- 36196665 Full text @ Chembiochem
Citation
Brown, W., Albright, S., Tsang, M., Deiters, A. (2022) Optogenetic Protein Cleavage in Zebrafish Embryos. Chembiochem : a European journal of chemical biology. 23(23):e202200297.
Abstract
A wide array of optogenetic tools is available that allow for precise spatiotemporal control over many cellular processes. These tools have been especially popular among zebrafish researchers who take advantage of the embryo's transparency. However, photocleavable optogenetic proteins have not been utilized in zebrafish. We demonstrate successful optical control of protein cleavage in embryos using PhoCl, a photocleavable fluorescent protein. This optogenetic tool offers temporal and spatial control over protein cleavage events, which we demonstrate in light-triggered protein translocation and apoptosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping