PUBLICATION
Dietary supplementation of exopolysaccharides from Lactobacillus rhamnosus GCC-3 improved the resistance of zebrafish against spring viremia of carp virus infection
- Authors
- Xie, M., Li, Y., Olsen, R.E., Ringø, E., Yang, Y., Zhang, Z., Ran, C., Zhou, Z.
- ID
- ZDB-PUB-220824-12
- Date
- 2022
- Source
- Frontiers in immunology 13: 968348 (Journal)
- Registered Authors
- Ran, Chao, Yang, Yalin, Zhang, Zhen, Zhou, Zhigang
- Keywords
- GCC-3 EPS, gut microbiota, spring viremia of carp virus, type I IFN, zebrafish
- MeSH Terms
-
- Animals
- Antiviral Agents/therapeutic use
- Carps*
- Dietary Supplements
- Fish Diseases*
- Interferon Type I*/therapeutic use
- Lacticaseibacillus rhamnosus*
- Rhabdoviridae
- Rhabdoviridae Infections*/veterinary
- Viremia
- Zebrafish
- PubMed
- 35990638 Full text @ Front Immunol
Citation
Xie, M., Li, Y., Olsen, R.E., Ringø, E., Yang, Y., Zhang, Z., Ran, C., Zhou, Z. (2022) Dietary supplementation of exopolysaccharides from Lactobacillus rhamnosus GCC-3 improved the resistance of zebrafish against spring viremia of carp virus infection. Frontiers in immunology. 13:968348.
Abstract
Spring viremia of carp virus (SVCV) can cause high mortality of fish. The aim of this study was to investigate the effects of Lactobacillus rhamnosus GCC-3 exopolysaccharides (GCC-3 EPS) on zebrafish (Danio rerio) infected with SVCV and elucidate the underlying mechanisms. Zebrafish were fed with a control diet or diet supplemented with 0.5% and 1% of GCC-3 EPS for 2 weeks. The results showed that supplementation of GCC-3 EPS significantly improved the survival rate of zebrafish compared with the control group. In addition, dietary 0.5% and 1% GCC-3 EPS significantly up-regulated the expression of genes related to type I interferon (IFN) antiviral immunity. Consistent with in vivo results, GCC-3 EPS significantly inhibited SVCV replication in zebrafish embryonic fibroblast (ZF4) cells while significantly increased the expression of type I IFN signaling pathway related genes. Furthermore, knocking down TANK-binding kinase 1 significantly blocked the antiviral effect of GCC-3 EPS. Dietary GCC-3 EPS improved gut microbiota, and the culture supernatant of GCC-3 EPS-associated microbiota significantly inhibited SVCV replication in ZF4 cells compared with the control-microbiota counterpart. In conclusion, our results indicate that dietary GCC-3 EPS can improve the resistance of zebrafish against SVCV infection, and the mechanism may involve enhanced type I interferon signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping