PUBLICATION
Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cells
- Authors
- Kuromiya, K., Aoki, K., Ishibashi, K., Yotabun, M., Sekai, M., Tanimura, N., Iijima, S., Ishikawa, S., Kamasaki, T., Akieda, Y., Ishitani, T., Hayashi, T., Toda, S., Yokoyama, K., Lee, C.G., Usami, I., Inoue, H., Takigawa, I., Gauquelin, E., Sugimura, K., Hino, N., Fujita, Y.
- ID
- ZDB-PUB-220714-10
- Date
- 2022
- Source
- Cell Reports 40: 111078 (Journal)
- Registered Authors
- Akieda, Yuki
- Keywords
- AHNAK2, CP: Cell biology, CP: Developmental biology, Cdc42, RasV12, TRPC1, apical extrusion, calcium sparks, cell competition, epithelia, phospho-SILAC screening, zebrafish larvae
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Signaling*
- Cell Movement
- Dogs
- Epithelial Cells/metabolism
- Madin Darby Canine Kidney Cells
- Zebrafish*/metabolism
- PubMed
- 35830802 Full text @ Cell Rep.
Citation
Kuromiya, K., Aoki, K., Ishibashi, K., Yotabun, M., Sekai, M., Tanimura, N., Iijima, S., Ishikawa, S., Kamasaki, T., Akieda, Y., Ishitani, T., Hayashi, T., Toda, S., Yokoyama, K., Lee, C.G., Usami, I., Inoue, H., Takigawa, I., Gauquelin, E., Sugimura, K., Hino, N., Fujita, Y. (2022) Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cells. Cell Reports. 40:111078.
Abstract
In vertebrates, newly emerging transformed cells are often apically extruded from epithelial layers through cell competition with surrounding normal epithelial cells. However, the underlying molecular mechanism remains elusive. Here, using phospho-SILAC screening, we show that phosphorylation of AHNAK2 is elevated in normal cells neighboring RasV12 cells soon after the induction of RasV12 expression, which is mediated by calcium-dependent protein kinase C. In addition, transient upsurges of intracellular calcium, which we call calcium sparks, frequently occur in normal cells neighboring RasV12 cells, which are mediated by mechanosensitive calcium channel TRPC1 upon membrane stretching. Calcium sparks then enhance cell movements of both normal and RasV12 cells through phosphorylation of AHNAK2 and promote apical extrusion. Moreover, comparable calcium sparks positively regulate apical extrusion of RasV12-transformed cells in zebrafish larvae as well. Hence, calcium sparks play a crucial role in the elimination of transformed cells at the early phase of cell competition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping