In vivo anti-inflammatory and antioxidant effects of microbial polysaccharides extracted from Euganean therapeutic muds
- Zampieri, R.M., Adessi, A., Caldara, F., De Philippis, R., Valle, L.D., La Rocca, N.
- International journal of biological macromolecules 209(Pt B): 1710-1719 (Journal)
- Registered Authors
- Dalla Valle, Luisa, Zampieri, Raffaella Margherita
- Cyanobacteria, Inflammation, Microbiota, Polysaccharides, Thermal muds, Zebrafish
- MeSH Terms
- Anti-Inflammatory Agents/pharmacology
- Copper Sulfate
- Oxidative Stress
- Polysaccharides/therapeutic use
- 35483514 Full text @ Int. J. Biol. Macromol.
Zampieri, R.M., Adessi, A., Caldara, F., De Philippis, R., Valle, L.D., La Rocca, N. (2022) In vivo anti-inflammatory and antioxidant effects of microbial polysaccharides extracted from Euganean therapeutic muds. International journal of biological macromolecules. 209(Pt B):1710-1719.
Therapeutic thermal mud produced by spas of the Euganean Thermal District (Italy) is used as a treatment for arthro-rheumatic diseases. Its production involves the growth of a specific microbiota embedded in a polysaccharidic matrix. Polysaccharides (Microbial-PolySaccharides, M-PS) released in the mud by the resident microorganisms were extracted and analyzed. The monosaccharidic composition analysis showed the presence of galacturonic acid, mannose, xylose, ribose and glucose and a high percentage of sulfated groups in the polymers. To assess their involvement in the therapeutic efficacy of the mud, the M-PS were tested using the model organism zebrafish (Danio rerio). The anti-inflammatory and antioxidant activities were evaluated after confirming the lack of toxic effects during development. Inflammatory state was induced chemically with copper sulfate, or through tail fin amputation procedure and UVB exposure. Recovery from inflammatory condition after exposure to M-PS was always observed with specific morphometric analyses, and further supported by qPCR. Genes linked with the inflammatory and oxidative stress response were investigated confirming the M-PS treatment's efficacy.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes