PUBLICATION
The Thermal Stability of the Collagen Triple Helix Is Tuned According to the Environmental Temperature
- Authors
- Fujii, K.K., Taga, Y., Takagi, Y.K., Masuda, R., Hattori, S., Koide, T.
- ID
- ZDB-PUB-220227-6
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(4): (Journal)
- Registered Authors
- Keywords
- collagen, environmental temperature, post-translational modification, thermal stability, triple helix
- MeSH Terms
-
- Animals
- Cell Line
- Collagen/chemistry*
- Collagen/metabolism
- Fibroblasts/metabolism
- Protein Conformation, alpha-Helical
- Protein Processing, Post-Translational
- Protein Stability
- Temperature
- Zebrafish
- PubMed
- 35216155 Full text @ Int. J. Mol. Sci.
Citation
Fujii, K.K., Taga, Y., Takagi, Y.K., Masuda, R., Hattori, S., Koide, T. (2022) The Thermal Stability of the Collagen Triple Helix Is Tuned According to the Environmental Temperature. International Journal of Molecular Sciences. 23(4):.
Abstract
Triple helix formation of procollagen occurs in the endoplasmic reticulum (ER) where the single-stranded α-chains of procollagen undergo extensive post-translational modifications. The modifications include prolyl 4- and 3-hydroxylations, lysyl hydroxylation, and following glycosylations. The modifications, especially prolyl 4-hydroxylation, enhance the thermal stability of the procollagen triple helix. Procollagen molecules are transported to the Golgi and secreted from the cell, after the triple helix is formed in the ER. In this study, we investigated the relationship between the thermal stability of the collagen triple helix and environmental temperature. We analyzed the number of collagen post-translational modifications and thermal melting temperature and α-chain composition of secreted type I collagen in zebrafish embryonic fibroblasts (ZF4) cultured at various temperatures (18, 23, 28, and 33 °C). The results revealed that thermal stability and other properties of collagen were almost constant when ZF4 cells were cultured below 28 °C. By contrast, at a higher temperature (33 °C), an increase in the number of post-translational modifications and a change in α-chain composition of type I collagen were observed; hence, the collagen acquired higher thermal stability. The results indicate that the thermal stability of collagen could be autonomously tuned according to the environmental temperature in poikilotherms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping