PUBLICATION
MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis
- Authors
- Tsai, Y.W., Jeng, K.S., He, M.K., Hsieh, Y.W., Lai, H.H., Lai, C.Y., Huang, C.C., Chang, C.F., Huang, C.T., Her, G.M.
- ID
- ZDB-PUB-211230-9
- Date
- 2021
- Source
- Cells 10(12): (Journal)
- Registered Authors
- Her, Guor Muor
- Keywords
- fibrogenesis, hepatic steatosis, liver cancer, obesity, steatohepatitis
- MeSH Terms
-
- Adipogenesis/genetics
- Animals
- Animals, Genetically Modified/genetics
- Carcinoma, Hepatocellular/complications
- Carcinoma, Hepatocellular/drug therapy
- Carcinoma, Hepatocellular/genetics*
- Carcinoma, Hepatocellular/pathology
- Doxycycline/pharmacology
- Gene Expression Regulation, Neoplastic/drug effects
- Humans
- Lipogenesis/drug effects
- Liver/metabolism
- Liver Neoplasms/complications
- Liver Neoplasms/drug therapy
- Liver Neoplasms/genetics*
- Liver Neoplasms/pathology
- Non-alcoholic Fatty Liver Disease/complications
- Non-alcoholic Fatty Liver Disease/drug therapy
- Non-alcoholic Fatty Liver Disease/genetics
- Non-alcoholic Fatty Liver Disease/pathology
- Obesity/complications
- Obesity/drug therapy
- Obesity/genetics*
- Obesity/pathology
- Receptors, Androgen/genetics*
- Repressor Proteins/genetics*
- Signal Transduction/genetics
- Zebrafish/genetics
- PubMed
- 34943942 Full text @ Cells
Citation
Tsai, Y.W., Jeng, K.S., He, M.K., Hsieh, Y.W., Lai, H.H., Lai, C.Y., Huang, C.C., Chang, C.F., Huang, C.T., Her, G.M. (2021) MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis. Cells. 10(12):.
Abstract
Obesity is closely linked to metabolic diseases, particularly non-alcoholic steatohepatitis (NASH) or non-alcoholic fatty liver disease (NAFLD), ultimately leading to hepatocellular carcinoma (HCC). However, the molecular mechanisms of NASH-associated HCC (NAHCC) remain elusive. To explore the impact of Max dimerization protein 3 (MXD3), a transcription factor that regulates several cellular functions in disorders associated with metabolic diseases, we conditionally expressed Mxd3 proteins using Tet-on mxd3 transgenic zebrafish (MXs) with doxycycline (MXs + Dox) or without doxycycline (MXs - Dox) treatment. Overexpression of global MXD3 (gMX) or hepatic Mxd3 (hMX) was associated with obesity-related NAFLD pathophysiology in gMX + Dox, and liver fibrosis and HCC in hMX + Dox. Oil Red O (ORO)-stained signals were seen in intravascular blood vessels and liver buds of larval gMX + Dox, indicating that Mxd3 functionally promotes lipogenesis. The gMX + Dox-treated young adults exhibited an increase in body weight and visceral fat accumulation. The hMX + Dox-treated young adults showed normal body characteristics but exhibited liver steatosis and NASH-like phenotypes. Subsequently, steatohepatitis, liver fibrosis, and NAHCC were found in 6-month-old gMX + Dox adults compared with gMX - Dox adults at the same stage. Overexpression of Mxd3 also enhanced AR expression accompanied by the increase of AR-signaling pathways resulting in hepatocarcinogenesis in males. Our results demonstrate that global actions of Mxd3 are central to the initiation of obesity in the gMX zebrafish through their effects on adipogenesis and that MXD3 could serve as a therapeutic target for obesity-associated liver diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping