PUBLICATION
Bucky Ball Is a Novel Zebrafish Vasa ATPase Activator
- Authors
- Perera, R.P., Shaikhqasem, A., Rostam, N., Dickmanns, A., Ficner, R., Tittmann, K., Dosch, R.
- ID
- ZDB-PUB-211025-31
- Date
- 2021
- Source
- Biomolecules 11(10): (Journal)
- Registered Authors
- Dosch, Roland
- Keywords
- ATPase activity, Bucky ball, Drosophila, Oskar, Vasa, germline, germplasm, primordial germ cells, zebrafish
- MeSH Terms
-
- Adenosine Triphosphatases/genetics
- Animals
- Cytoplasm
- DEAD-box RNA Helicases/genetics*
- Germ Cells/growth & development
- Germ Cells/metabolism
- Oocytes/growth & development
- Oocytes/metabolism
- Protein Binding/genetics
- RNA/genetics
- Ribonucleoproteins/genetics*
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- PubMed
- 34680140 Full text @ Biomolecules
Citation
Perera, R.P., Shaikhqasem, A., Rostam, N., Dickmanns, A., Ficner, R., Tittmann, K., Dosch, R. (2021) Bucky Ball Is a Novel Zebrafish Vasa ATPase Activator. Biomolecules. 11(10):.
Abstract
Many multicellular organisms specify germ cells during early embryogenesis by the inheritance of ribonucleoprotein (RNP) granules known as germplasm. However, the role of complex interactions of RNP granules during germ cell specification remains elusive. This study characterizes the interaction of RNP granules, Buc, and zebrafish Vasa (zfVasa) during germ cell specification. We identify a novel zfVasa-binding motif (Buc-VBM) in Buc and a Buc-binding motif (zfVasa-BBM) in zfVasa. Moreover, we show that Buc and zfVasa directly bind in vitro and that this interaction is independent of the RNA. Our circular dichroism spectroscopy data reveal that the intrinsically disordered Buc-VBM peptide forms alpha-helices in the presence of the solvent trifluoroethanol. Intriguingly, we further demonstrate that Buc-VBM enhances zfVasa ATPase activity, thereby annotating the first biochemical function of Buc as a zfVasa ATPase activator. Collectively, these results propose a model in which the activity of zfVasa is a central regulator of primordial germ cell (PGC) formation and is tightly controlled by the germplasm organizer Buc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping