PUBLICATION
Corpuscles of Stannius development requires FGF signaling
- Authors
- Klingbeil, K., Nguyen, T., Fahrner, A., Guthmann, C., Wang, H., Schoels, M., Lilienkamp, M., Franz, H., Eckert, P., Walz, G., Yakulov, T.A.
- ID
- ZDB-PUB-211022-40
- Date
- 2021
- Source
- Developmental Biology 481: 160-171 (Journal)
- Registered Authors
- Keywords
- Corpuscles of Stannius, Cpe, Development, Fibroblast growth factor (FGF), Kidney, Pronephros, Wnt signaling, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation*
- Endocrine Glands/embryology*
- Fibroblast Growth Factors*/genetics
- Fibroblast Growth Factors*/metabolism
- Glycoproteins/genetics
- Glycoproteins/metabolism
- Pronephros/embryology*
- Wnt Signaling Pathway*
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 34666023 Full text @ Dev. Biol.
Citation
Klingbeil, K., Nguyen, T., Fahrner, A., Guthmann, C., Wang, H., Schoels, M., Lilienkamp, M., Franz, H., Eckert, P., Walz, G., Yakulov, T.A. (2021) Corpuscles of Stannius development requires FGF signaling. Developmental Biology. 481:160-171.
Abstract
The corpuscles of Stannius (CS) represent a unique endocrine organ of teleostean fish that secrets stanniocalcin-1 (Stc1) to maintain calcium homeostasis. Appearing at 20-25 somite stage in the distal zebrafish pronephros, stc1-expressing cells undergo apical constriction, and are subsequently extruded to form a distinct gland on top of the distal pronephric tubules at 50 h post fertilization (hpf). Several transcription factors (e.g. Hnf1b, Irx3b, Tbx2a/b) and signaling pathways (e.g. Notch) control CS development. We report now that Fgf signaling is required to commit tubular epithelial cells to differentiate into stc1-expressing CS cells. Inhibition of Fgf signaling by SU5402, dominant-negative Fgfr1, or depletion of fgf8a prevented CS formation and stc1 expression. Ablation experiments revealed that CS have the ability to partially regenerate via active cell migration involving extensive filopodia and lamellipodia formation. Activation of Wnt signaling curtailed stc1 expression, but had no effect on CS formation. Thus, our observations identify Fgf signaling as a crucial component of CS cell fate commitment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping