PUBLICATION
Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis
- Authors
- Tanoue, A., Katayama, K., Ito, Y., Joh, K., Toda, M., Yasuma, T., D'Alessandro-Gabazza, C.N., Kawachi, H., Yan, K., Ito, M., Gabazza, E.C., Tryggvason, K., Dohi, K.
- ID
- ZDB-PUB-211022-3
- Date
- 2021
- Source
- Scientific Reports 11: 20556 (Journal)
- Registered Authors
- Tryggvason, Karlynn
- Keywords
- none
- MeSH Terms
-
- Animals
- Carrier Proteins/genetics*
- Cells, Cultured
- Glomerulosclerosis, Focal Segmental/blood
- Glomerulosclerosis, Focal Segmental/genetics*
- Glomerulosclerosis, Focal Segmental/pathology
- Humans
- Membrane Proteins/genetics*
- Mice, Knockout
- Podocytes/ultrastructure*
- PubMed
- 34654837 Full text @ Sci. Rep.
Citation
Tanoue, A., Katayama, K., Ito, Y., Joh, K., Toda, M., Yasuma, T., D'Alessandro-Gabazza, C.N., Kawachi, H., Yan, K., Ito, M., Gabazza, E.C., Tryggvason, K., Dohi, K. (2021) Podocyte-specific Crb2 knockout mice develop focal segmental glomerulosclerosis. Scientific Reports. 11:20556.
Abstract
Crb2 is a cell polarity-related type I transmembrane protein expressed in the apical membrane of podocytes. Knockdown of crb2 causes glomerular permeability defects in zebrafish, and its complete knockout causes embryonic lethality in mice. There are also reports of Crb2 mutations in patients with steroid-resistant nephrotic syndrome, although the precise mechanism is unclear. The present study demonstrated that podocyte-specific Crb2 knockout mice develop massive albuminuria and microhematuria 2-month after birth and focal segmental glomerulosclerosis and tubulointerstitial fibrosis with hemosiderin-laden macrophages at 6-month of age. Transmission and scanning electron microscopic studies demonstrated injury and foot process effacement of podocytes in 6-month aged podocyte-specific Crb2 knockout mice. The number of glomerular Wt1-positive cells and the expressions of Nphs2, Podxl, and Nphs1 were reduced in podocyte-specific Crb2 knockout mice compared to negative control mice. Human podocytes lacking CRB2 had significantly decreased F-actin positive area and were more susceptible to apoptosis than their wild-type counterparts. Overall, this study's results suggest that the specific deprivation of Crb2 in podocytes induces altered actin cytoskeleton reorganization associated with dysfunction and accelerated apoptosis of podocytes that ultimately cause focal segmental glomerulosclerosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping