PUBLICATION
Mechanical role of actinotrichia in shaping the caudal fin of zebrafish
- Authors
- Nakagawa, H., Kuroda, J., Aramaki, T., Kondo, S.
- ID
- ZDB-PUB-210922-3
- Date
- 2021
- Source
- Developmental Biology 481: 52-63 (Journal)
- Registered Authors
- Kondo, Shigeru
- Keywords
- Actinotrichia, Collagen fiber, Fin formation, Zebrafish, collagen9a1c (col9a1c)
- MeSH Terms
-
- Animal Fins/embryology*
- Animals
- Collagen Type IX/deficiency*
- Collagen Type IX/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockout Techniques
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/deficiency*
- Zebrafish Proteins/metabolism
- PubMed
- 34537221 Full text @ Dev. Biol.
Citation
Nakagawa, H., Kuroda, J., Aramaki, T., Kondo, S. (2021) Mechanical role of actinotrichia in shaping the caudal fin of zebrafish. Developmental Biology. 481:52-63.
Abstract
Spear-like collagen complexes, known as actinotrichia, underlie the epidermal cell layer in the tip of teleost fins and are known to contribute toward fin formation; however, their specific role remains largely unclear. In this study, we investigated of actinotrichia in the role of caudal fin formation by generating collagen9a1c (col9a1c)-knockout zebrafish. Although actinotrichia were initially produced normally and aligned correctly in the knockout fish, the number of actinotrichia decreased as the fish grew and their alignment became disordered. Simultaneously, the fin tip gradually shortened in the dorsal-ventral direction and the entire fin became oval-shaped, while the fin-rays rarely bifurcated and instead underwent fusion, suggesting that actinotrichia are essential for spreading fins dorsoventrally. Furthermore, the epithelial cells that are usually thinly spread in normal fish became spherical in the knockout fish, reducing the area covered by each cell and thus the area of the fin tip. Together, these findings suggest that the tight alignment of actinotrichia provides physical support in the dorsal-ventral direction that allows caudal fins to expand in a triangular-shape.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping