PUBLICATION
BMP and Notch Signaling Pathways differentially regulate Cardiomyocyte Proliferation during Ventricle Regeneration
- Authors
- Wang, W., Hu, Y.F., Pang, M., Chang, N., Yu, C., Li, Q., Xiong, J.W., Peng, Y., Zhang, R.
- ID
- ZDB-PUB-210710-3
- Date
- 2021
- Source
- International journal of biological sciences 17: 2157-2166 (Journal)
- Registered Authors
- Zhang, Ruilin
- Keywords
- BMP signaling, Notch signaling, cardiomyocyte proliferation, cell-cycle arrest, heart regeneration
- MeSH Terms
-
- Animals
- Bone Morphogenetic Protein Receptors/metabolism*
- Cell Cycle Checkpoints
- Cell Proliferation
- Heart/physiology*
- Myocytes, Cardiac/cytology*
- Myocytes, Cardiac/metabolism
- Receptors, Notch/metabolism*
- Regeneration*
- Signal Transduction*
- Ubiquitination
- Zebrafish/physiology
- PubMed
- 34239346 Full text @ Int. J. Biol. Sci.
Citation
Wang, W., Hu, Y.F., Pang, M., Chang, N., Yu, C., Li, Q., Xiong, J.W., Peng, Y., Zhang, R. (2021) BMP and Notch Signaling Pathways differentially regulate Cardiomyocyte Proliferation during Ventricle Regeneration. International journal of biological sciences. 17:2157-2166.
Abstract
Adult mammalian hearts show limited capacity to proliferate after injury, while zebrafish are capable to completely regenerate injured hearts through the proliferation of spared cardiomyocytes. BMP and Notch signaling pathways have been implicated in cardiomyocyte proliferation during zebrafish heart regeneration. However, the molecular mechanism underneath this process as well as the interaction between these two pathways remains to be further explored. In this study we showed BMP signaling was activated after ventricle ablation and acted epistatic downstream of Notch signaling. Inhibition of both signaling pathways differentially influenced ventricle regeneration and cardiomyocyte proliferation, as revealed by time-lapse analysis using a cardiomyocyte-specific FUCCI (fluorescent ubiquitylation-based cell cycle indicator) system. Further experiments revealed that inhibition of BMP and Notch signaling led to cell-cycle arrest at different phases. Overall, our results shed light on the interaction between BMP and Notch signaling pathways and their functions in cardiomyocyte proliferation during cardiac regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping