PUBLICATION
Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems
- Authors
- Binns, T.C., Ayala, A.X., Grimm, J.B., Tkachuk, A.N., Castillon, G.A., Phan, S., Zhang, L., Brown, T.A., Liu, Z., Adams, S.R., Ellisman, M.H., Koyama, M., Lavis, L.D.
- ID
- ZDB-PUB-210707-15
- Date
- 2020
- Source
- Cell chemical biology 27: 1063-1072.e7 (Journal)
- Registered Authors
- Keywords
- HaloTag, Janelia Fluor, cell ablation, electron microscopy, fluorescence, photochemistry, photosensitizer, reactive oxygen species, rhodamine
- MeSH Terms
-
- 3,3'-Diaminobenzidine/chemistry
- Animals
- Animals, Genetically Modified/metabolism
- Cell Line, Tumor
- Drug Design*
- Humans
- Larva/metabolism
- Ligands
- Light
- Microphthalmia-Associated Transcription Factor/genetics
- Microphthalmia-Associated Transcription Factor/metabolism
- Microscopy, Electron
- Neurons/chemistry
- Neurons/metabolism
- Photosensitizing Agents/chemistry*
- Photosensitizing Agents/metabolism
- Quantum Theory
- Rhodamines/chemistry
- Singlet Oxygen/chemistry
- Singlet Oxygen/metabolism
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32698018 Full text @ Cell Chem Biol
Citation
Binns, T.C., Ayala, A.X., Grimm, J.B., Tkachuk, A.N., Castillon, G.A., Phan, S., Zhang, L., Brown, T.A., Liu, Z., Adams, S.R., Ellisman, M.H., Koyama, M., Lavis, L.D. (2020) Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems. Cell chemical biology. 27:1063-1072.e7.
Abstract
Light-mediated chemical reactions are powerful methods for manipulating and interrogating biological systems. Photosensitizers, compounds that generate reactive oxygen species upon excitation with light, can be utilized for numerous biological experiments, but the repertoire of bioavailable photosensitizers is limited. Here, we describe the synthesis, characterization, and utility of two photosensitizers based upon the widely used rhodamine scaffold and demonstrate their efficacy for chromophore-assisted light inactivation, cell ablation in culture and in vivo, and photopolymerization of diaminobenzidine for electron microscopy. These chemical tools will facilitate a broad range of applications spanning from targeted destruction of proteins to high-resolution imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping