PUBLICATION
Effects of sulfometuron-methyl on zebrafish at early developmental stages
- Authors
- Yuan, W., Xu, Z., Wei, Y., Lu, W., Jia, K., Guo, J., Meng, Y., Peng, Y., Wu, Z., Zhu, Z., Ma, F., Wei, F., Tian, G., Liu, Z., Luo, Q., Ma, J., Zhang, H., Liu, W., Lu, H.
- ID
- ZDB-PUB-210604-4
- Date
- 2021
- Source
- Ecotoxicology and environmental safety 220: 112385 (Journal)
- Registered Authors
- Keywords
- Cell apoptosis, Immunotoxicity, Locomotor behavior, Oxidative stress, Sulfometuron-methyl, Zebrafish model
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Catalase/metabolism
- Cytokines/genetics
- Cytokines/metabolism
- Embryo, Nonmammalian/drug effects*
- Gene Expression Regulation, Developmental/drug effects
- Herbicides/toxicity*
- Larva/drug effects
- Oxidative Stress/drug effects
- Reactive Oxygen Species
- Sulfonylurea Compounds/toxicity*
- Superoxide Dismutase/metabolism
- Water Pollutants, Chemical/toxicity
- Zebrafish/growth & development*
- PubMed
- 34082241 Full text @ Ecotoxicol. Environ. Saf.
- CTD
- 34082241
Citation
Yuan, W., Xu, Z., Wei, Y., Lu, W., Jia, K., Guo, J., Meng, Y., Peng, Y., Wu, Z., Zhu, Z., Ma, F., Wei, F., Tian, G., Liu, Z., Luo, Q., Ma, J., Zhang, H., Liu, W., Lu, H. (2021) Effects of sulfometuron-methyl on zebrafish at early developmental stages. Ecotoxicology and environmental safety. 220:112385.
Abstract
Sulfometuron methyl (SM) is a widely used herbicide and thus leading to accumulation in the environment. The toxicity assessments of SM in model organisms are currently rare. In the present study, zebrafish were utilized for evaluating the detrimental effects of SM in aquatic vertebrates. Zebrafish embryos were exposed to 0, 10, 20, and 40 mg/L SM from 5.5 to 72 h post-fertilization (hpf), respectively. Consequently, SM exposure resulted in increasing the mortality rate and reducing hatching rate in larval zebrafish at 10, 20, and 40 mg/L SM-treated groups. The reduced numbers of immune cells (neutrophils and macrophages) were observed after SM exposure by a dose-dependent manner. The inflammatory responses (TLR4, MYD88, IL-1β, IL-6, IL-8, IFN-γ, IL-10, and TGF-β) were measured to estimate immune responses. Anti-inflammatory factors (IL-10 and TGF-β) were down-regulated in all the treated groups and significantly altered at 40 mg/L exposure group. Additionally, behavioral tests suggested that SM treatment significantly increased the total distance, average speed, and maximum acceleration of larval zebrafish during light-dark transition and subsequently enzymology test displayed the same trend to locomotor behaviors. The content significantly increased in oxidative stress, as reflected in ROS level in all the treated groups. The numbers of cell apoptosis were significantly increased at 20, and 40 mg/L and the highest concentration group induced the substantial increment (P < 0.001) of apoptosis-related genes including p53, Bax/Bcl-2, caspase-9, and caspase-3. In summary, our results demonstrated that exposure to SM caused toxicity of development, immune system, locomotor behavior, oxidative stress, and cell apoptosis at the early developmental stages of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping