PUBLICATION
The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development
- Authors
- Fittipaldi, R., Floris, P., Proserpio, V., Cotelli, F., Beltrame, M., Caretti, G.
- ID
- ZDB-PUB-210603-22
- Date
- 2021
- Source
- Cells 10(5): (Journal)
- Registered Authors
- Beltrame, Monica, Cotelli, Franco
- Keywords
- SMYD3, development, embryonic stem cells, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation*
- Cell Line
- Cell Lineage
- Embryonic Development
- Gene Expression Regulation, Developmental
- Histone-Lysine N-Methyltransferase/genetics
- Histone-Lysine N-Methyltransferase/metabolism*
- Mice
- Mouse Embryonic Stem Cells/enzymology*
- Time Factors
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 34069776 Full text @ Cells
Citation
Fittipaldi, R., Floris, P., Proserpio, V., Cotelli, F., Beltrame, M., Caretti, G. (2021) The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development. Cells. 10(5):.
Abstract
SMYD3 (SET and MYND domain containing protein 3) is a methylase over-expressed in cancer cells and involved in oncogenesis. While several studies uncovered key functions for SMYD3 in cancer models, the SMYD3 role in physiological conditions has not been fully elucidated yet. Here, we dissect the role of SMYD3 at early stages of development, employing mouse embryonic stem cells (ESCs) and zebrafish as model systems. We report that SMYD3 depletion promotes the induction of the mesodermal pattern during in vitro differentiation of ESCs and is linked to an upregulation of cardiovascular lineage markers at later stages. In vivo, smyd3 knockdown in zebrafish favors the upregulation of mesendodermal markers during zebrafish gastrulation. Overall, our study reveals that SMYD3 modulates levels of mesendodermal markers, both in development and in embryonic stem cell differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping