PUBLICATION
Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration
- Authors
- Zeng, C.W., Kamei, Y., Shigenobu, S., Sheu, J.C., Tsai, H.J.
- ID
- ZDB-PUB-210225-1
- Date
- 2021
- Source
- Open Biology 11: 200304 (Journal)
- Registered Authors
- Tsai, Huai-Jen
- Keywords
- axon, caveolin-1, motoneuron, neuronal regeneration, spinal cord injury, transgenic zebrafish
- MeSH Terms
-
- Animals
- Caveolin 1/genetics
- Caveolin 1/metabolism*
- Cells, Cultured
- Motor Neurons/cytology
- Motor Neurons/metabolism
- Neural Stem Cells/cytology
- Neural Stem Cells/metabolism
- Neuronal Outgrowth
- Spinal Cord Injuries/metabolism
- Spinal Cord Injuries/physiopathology
- Spinal Cord Regeneration*
- Up-Regulation
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33622104 Full text @ Open Biol.
Citation
Zeng, C.W., Kamei, Y., Shigenobu, S., Sheu, J.C., Tsai, H.J. (2021) Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration. Open Biology. 11:200304.
Abstract
The extent of cellular heterogeneity involved in neuronal regeneration after spinal cord injury (SCI) remains unclear. Therefore, we established stress-responsive transgenic zebrafish embryos with SCI. As a result, we found an SCI-induced cell population, termed SCI stress-responsive regenerating cells (SrRCs), essential for neuronal regeneration post-SCI. SrRCs were mostly composed of subtypes of radial glia (RGs-SrRCs) and neuron stem/progenitor cells (NSPCs-SrRCs) that are able to differentiate into neurons, and they formed a bridge across the lesion and connected with neighbouring undamaged motor neurons post-SCI. Compared to SrRCs at the caudal side of the SCI site (caudal-SrRCs), rostral-SrRCs participated more actively in neuronal regeneration. After RNA-seq analysis, we discovered that caveolin 1 (cav1) was significantly upregulated in rostral-SrRCs and that cav1 was responsible for the axonal regrowth and regenerative capability of rostral-SrRCs. Collectively, we define a specific SCI-induced cell population, SrRCs, involved in neuronal regeneration, demonstrate that rostral-SrRCs exhibit higher neuronal differentiation capability and prove that cav1 is predominantly expressed in rostral-SrRCs, playing a major role in neuronal regeneration after SCI.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping