ZFIN ID: ZDB-PERS-010509-1
Tsai, Huai-Jen
Email: hjtsai@ntu.edu.tw
URL:
Affiliation: Tsai Laboratory
Address: Insitute of Molecular and Cellular Biology College of Life Sciences National Taiwan University 1, Roosevelt Road, Sec. 4 Taipei, Taiwan
Country: Taiwan
Phone: 886-2-2364-1705 or 886-2-3366-2487
Fax: 886-2-2363-8483
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS
Molecular Biology and Marine Biotechnology


PUBLICATIONS
Lin, C.Y., He, J.Y., Zeng, C.W., Loo, M.R., Chang, W.Y., Zhang, P.H., Tsai, H.J. (2017) microRNA-206 modulates an Rtn4a/Cxcr4a/Thbs3a axis in newly forming somites to maintain and stabilize the somite boundary formation of zebrafish embryos. Open Biology. 7(7)
Fu, C.Y., Wang, P.C., Tsai, H.J. (2017) Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis. Nucleic acids research. 45:2423-2437
Zeng, C.W., Kamei, Y., Wang, C.T., Tsai, H.J. (2016) Subtypes of hypoxia-responsive cells differentiate into neurons in spinal cord of zebrafish embryos after hypoxic stress. Biology of the cell. 108(12):357-377
Lin, C.Y., Chiang, C.Y., Tsai, H.J. (2016) Zebrafish and Medaka: new model organisms for modern biomedical research. Journal of Biomedical Science. 23:19
Yu, W.H., Ho, Y.L., Huang, P.T., Chu, S.L., Tsai, H.J., Liou, H.H. (2016) The Phosphorylation State of GSK3β Serine 9 Correlated to the Development of Valproic Acid-Associated Fetal Cardiac Teratogenicity, Fetal VPA Syndrome, Rescued by Folic Acid Administration. Cardiovascular Toxicology. 16(1):34-45
Lee, H.C., Su, M.Y., Lo, H.C., Wu, C.C., Hu, J.R., Lo, D.M., Chao, T.Y., Tsai, H.J., Dai, M.S. (2015) Cancer metastasis and EGFR signaling is suppressed by Amiodarone-induced Versican V2. Oncotarget. 6(40):42976-87
Lee, H.C., Lo, H.C., Lo, D.M., Su, M.Y., Hu, J.R., Wu, C.C., Chang, S.N., Dai, M.S., Tsai, C.T., Tsai, H.J. (2015) Amiodarone Induces Overexpression of Similar to Versican b to Repress the EGFR/Gsk3b/Snail Signaling Axis during Cardiac Valve Formation of Zebrafish Embryos. PLoS One. 10:e0144751
Lin, C.Y., Huang, H.Y., Lu, P.N., Lin, C.W., Lu, K.M., Tsai, H.J. (2015) Ras-Related Nuclear Protein is required for late developmental stages of retinal cells in zebrafish eyes. The International journal of developmental biology. 59:435-442
Lee, H.C., Lin, Y.Z., Lai, Y.T., Huang, W.J., Hu, J.R., Tsai, J.N., Tsai, H.J. (2014) Glycogen Synthase Kinase 3 beta in somites plays a role during the angiogenesis of zebrafish embryos. The FEBS journal. 281(19):4367-83
Chiang, C.Y., Chen, Y.L., and Tsai, H.J. (2014) Different Visible Colors and Green Fluorescence Were Obtained from the Mutated Purple Chromoprotein Isolated from Sea Anemone. Mar. Biotechnol.. 16(4):436-46
Candel, S., de Oliveira, S., López-Muñoz, A., García-Moreno, D., Espín-Palazón, R., Tyrkalska, S.D., Cayuela, M.L., Renshaw, S.A., Corbalán-Vélez, R., Vidal-Abarca, I., Tsai, H.J., Meseguer, J., Sepulcre, M.P., Mulero, V. (2014) Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation. PLoS Biology. 12:e1001855
Lee, H.C., Lu, P.N., Huang, H.L., Chu, C., Li, H.P., Tsai, H.J. (2014) Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants. PLoS One. 9:e90160
Lin, C.Y., Lee, H.C., Chen, H.C., Hsieh, C.C., and Tsai, H.J. (2013) Normal Function of Myf5 During Gastrulation Is Required for Pharyngeal Arch Cartilage Development in Zebrafish Embryos. Zebrafish. 10(4):486-99
Lin, C.Y., Chen, J.S., Loo, M.R., Hsiao, C.C., Chang, W.Y., and Tsai, H.J. (2013) MicroRNA-3906 Regulates Fast Muscle Differentiation through Modulating the Target Gene homer-1b in Zebrafish Embryos. PLoS One. 8(7):e70187
Lin, C.Y., Lee, H.C., Fu, C.Y., Ding, Y.Y., Chen, J.S., Lee, M.H., Huang, W.J., and Tsai, H.J. (2013) miR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos. Nature communications. 4:2829
Chen, Y.H., Hsu, R.J., Chen, T.Y., Huang, Y.K., Lee, H.C., Hu, S.C., Harn, H.J., Jeng, J.R., Sun, C.K., Lin, S.Z., and Tsai, H.J. (2012) The toxic effect of Amiodarone on valve formation in the developing heart of zebrafish embryos. Reproductive toxicology (Elmsford, N.Y.). 33(2):233-244
Tu, C.T., Yang, T.C., Huang, H.Y., and Tsai, H.J. (2012) Zebrafish arl6ip1 Is Required for Neural Crest Development during Embryogenesis. PLoS One. 7(3):e32899
Huang, H.Y., Liu, J.T., Yan, H.Y., and Tsai, H.J. (2012) Arl6ip1 Plays a Role in Proliferation during Zebrafish Retinogenesis. Cells, tissues, organs. 196(2):161-174
Lee, H.C., Chen, Y.J., Liu, Y.W., Lin, K.Y., Chen, S.W., Lin, C.Y., Lu, Y.C., Hsu, P.C., Lee, S.C., and Tsai, H.J. (2011) Transgenic zebrafish model to study translational control mediated by upstream open reading frame of human chop gene. Nucleic acids research. 39(20):e139
Chang, S.H., Lee, B.C., Chen, Y.D., Lee, Y.C., and Tsai, H.J. (2011) Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein. Transgenic Research. 20(5):1099-111
Hami, D., Grimes, A.C., Tsai, H.J., and Kirby, M.L. (2011) Zebrafish cardiac development requires a conserved secondary heart field. Development (Cambridge, England). 138(11):2389-2398
Hsu, R.J., Lin, C.C., Su, Y.F., and Tsai, H.J. (2011) dickkopf-3-related Gene Regulates the Expression of Zebrafish myf5 Gene through Phosphorylated p38a-dependent Smad4 Activity. The Journal of biological chemistry. 286(8):6855-6864
Hsu, R.J., and Tsai, H.J. (2011) Performing the Labeled microRNA Pull-Down (LAMP) Assay System: An Experimental Approach for High-Throughput Identification of microRNA-Target mRNAs. Meth. Mol. Biol.. 764:241-247
Hsu, R.J., Lin, C.Y., Hoi, H.S., Zheng, S.K., Lin, C.C., and Tsai, H.J. (2010) Novel intronic microRNA represses zebrafish myf5 promoter activity through silencing dickkopf-3 gene. Nucleic acids research. 38(13):4384-4393
Lin, C.Y., Yang, P.H., Kao, C.L., Huang, H.I., and Tsai, H.J. (2010) Transgenic zebrafish eggs containing bactericidal peptide is a novel food supplement enhancing resistance to pathogenic infection of fish. Fish & shellfish immunology. 28(3):419-427
Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W. (2010) Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development. Developmental Biology. 337(2):432-443
Lee, H.C., Tseng, W.A., Lo, F.Y., Liu, T.M., and Tsai, H.J. (2009) FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis. Developmental Biology. 336(2):232-245
Ohn, J., Tsai, H.J., and Liebling, M. (2009) Joint dynamic imaging of morphogenesis and function in the developing heart. Organogenesis. 5(4):166-173
Ho, Y.L., Lin, Y.H., Tsai, W.Y., Hsieh, F.J., and Tsai, H.J. (2009) Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy. Circulation journal : official journal of the Japanese Circulation Society. 73(9):1691-1697
Lin, C.Y., Chen, W.T., Lee, H.C., Yang, P.H., Yang, H.J., and Tsai, H.J. (2009) The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish. Developmental Biology. 331(2):152-166
Fu, C.Y., Lee, H.C., and Tsai, H.J. (2009) The Molecular Structures and Expression Patterns of Zebrafish Troponin I Genes. Gene Expression Patterns. 9(5):348-356
Hsu, R.J., Yang, H.J., and Tsai, H.J. (2009) Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs. Nucleic acids research. 37(10):e77
Huang, H.Y., Dai, E.S., Liu, J.T., Tu, C.T., Yang, T.C., and Tsai, H.J. (2009) The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Developmental dynamics : an official publication of the American Association of Anatomists. 238(1):232-240
Tu, C.T., Yang, T.C., and Tsai, H.J. (2009) Nkx2.7 and Nkx2.5 function redundantly and are required for cardiac morphogenesis of zebrafish embryos. PLoS One. 4(1):e4249
Grimes, A.C., Erwin, K.N., Stadt, H.A., Hunter, G.L., Gefroh, H.A., Tsai, H.J., and Kirby, M.L. (2008) PCB126 Exposure Disrupts Zebrafish Ventricular and Branchial but Not Early Neural Crest Development. Toxicological sciences : an official journal of the Society of Toxicology. 106(1):193-205
Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D. (2008) The orphan G protein-coupled receptor 161 is required for left-right patterning. Developmental Biology. 323(1):31-40
Lee, H.C., Tsai, J.N., Liao, P.Y., Tsai, W.Y., Lin, K.Y., Chuang, C.C., Sun, C.K., Chang, W.C., and Tsai, H.J. (2007) Glycogen synthase kinase 3alpha and 3beta have distinct functions during cardiogenesis of zebrafish embryo. BMC Developmental Biology. 7(1):93
Holtzman, N.G., Schoenebeck, J.J., Tsai, H.J., and Yelon, D. (2007) Endocardium is necessary for cardiomyocyte movement during heart tube assembly. Development (Cambridge, England). 134(13):2379-2386
Auman, H.J., Coleman, H., Riley, H.E., Olale, F., Tsai, H.J., and Yelon, D. (2007) Functional Modulation of Cardiac Form through Regionally Confined Cell Shape Changes. PLoS Biology. 5(3):e53
Ho, Y.L., Lin, Y.H., Tsai, I.J., Hsieh, F.J., and Tsai, H.J. (2007) In Vivo Assessment of Cardiac Morphology and Function in Heart-specific Green Fluorescent Zebrafish. Journal of the Formosan Medical Association = Taiwan yi zhi. 106(3):181-186
Chen, Y.H., Wang, Y.H., Chang, M.Y., Lin, C.Y., Weng, C.W., Westerfield, M., and Tsai, H.J. (2007) Multiple upstream modules regulate zebrafish myf5 expression. BMC Developmental Biology. 7(1):1
Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J. (2006) Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology. 299(2):594-608
Hu, C.Y., Yang, C.H., Chen, W.Y., Huang, C.J., Huang, H.Y., Chen, M.S., and Tsai, H.J. (2006) Egr1 gene knockdown affects embryonic ocular development in zebrafish. Molecular Vision. 12:1250-1258
Sabaawy, H.E., Azuma, M., Embree, L.J., Tsai, H.J., Starost, M.F., and Hickstein, D.D. (2006) TEL-AML1 transgenic zebrafish model of precursor B cell acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA. 103(41):15166-15171
Sato, M., Tsai, H.J., and Yost, H.J. (2006) Semaphorin3D regulates invasion of cardiac neural crest cells into the primary heart field. Developmental Biology. 298(1):12-21
Chen, S.Y., Hsieh, C.S., Chu, S.W., Lin, C.Y., Ko, C.Y., Chen, Y.C., Tsai, H.J.,Hu, C.H., and Sun, C.K. (2006) Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo. Journal of Biomedical Optics. 11(5):54022
Forouhar, A.S., Liebling, M., Hickerson, A., Nasiraei-Moghaddam, A., Tsai, H.J., Hove, J.R., Fraser, S.E., Dickinson, M.E., and Gharib, M. (2006) The embryonic vertebrate heart tube is a dynamic suction pump. Science (New York, N.Y.). 312(5774):751-753
Wang, Y.H., Chen, Y.H., Lu, J.H., Lin, Y.J., Chang, M.Y., and Tsai, H.J. (2006) Epidermis-restricted expression of zebrafish cytokeratin II is controlled by a -141/+85 minimal promoter, and cassette -141/-111 is essential for driving the tissue specificity. Differentiation; research in biological diversity. 74(4):186-194
Tsai, T.H., Lin, C.Y., Tsai, H.J., Chen, S.Y., Tai, S.P., Lin, K.H., and Sun, C.K. (2006) Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section. Optics letters. 31(7):930-932
Wang, Y.H., Chen, Y.H., Lin, Y.J., and Tsai, H.J. (2006) Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line. Gene Expression Patterns. 6(4):335-339
Lee, H.C., Huang, H.Y., Lin, C.Y., Chen, Y.H., and Tsai, H.J. (2006) Foxd3 mediates zebrafish myf5 expression during early somitogenesis. Developmental Biology. 290(2):359-372
Wang, Y.H., Chen, Y.H., Lu, J.H., and Tsai, H.J. (2005) A 23-amino Acid motif spanning the basic domain targets zebrafish myogenic regulatory factor myf5 into nucleolus. DNA and cell biology. 24(10):651-660
Huang, C.J., Jou, T.S., Ho, Y.L., Lee, W.H., Jeng, Y.T., Hsieh, F.J., and Tsai, H.J. (2005) Conditional expression of a myocardium-specific transgene in zebrafish transgenic lines. Developmental dynamics : an official publication of the American Association of Anatomists. 233(4):1294-1303
Sun, C.K., Chu, S.W., Chen, S.Y., Tsai, T.H., Liu, T.M., Lin, C.Y., and Tsai, H.J. (2004) Higher harmonic generation microscopy for developmental biology. Journal of structural biology. 147(1):19-30
Lin, C.Y., Chen, Y.H., Lee, H.C., and Tsai, H.J. (2004) Novel cis-element in intron 1 represses somite expression of zebrafish myf-5. Gene. 334:63-72
Hsiao, C.D., Ekker, M., and Tsai, H.J. (2003) Skin-specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 228(4):745-750
Shu, X., Cheng, K., Patel, N., Chen, F., Joseph, E., Tsai, H.-J., and Chen, J.-N. (2003) Na,K-ATPase is essential for embryonic heart development in the zebrafish. Development (Cambridge, England). 130:6165-6173
Hsiao, C.-D. and Tsai, H.-J. (2003) Transgenic zebrafish with fluorescent germ cell: a useful tool to visualize germ cell proliferation and juvenile hermaphroditism in vivo. Developmental Biology. 262:313-323
Raya, A., Koth, C.M., Büscher, D., Kawakami, Y., Itoh, T., Raya, R.M., Sternick, G., Tsai, H.J., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C. (2003) Activation of Notch signaling pathway precedes heart regeneration in zebrafish. Proc. Natl. Acad. Sci. USA. (Suppl. 1) 100:11889-11895
Chen, Y.-H., Lee, H.-C., Liu, C.-F., Lin, C.-Y., and Tsai, H.-J. (2003) Novel regulatory sequence -82/-62 functions as a key element to drive the somite-specificity of zebrafish myf-5. Developmental dynamics : an official publication of the American Association of Anatomists. 228(1):41-50
Huang, C.-J., Tu, C.-T., Hsiao, C.-D., Hsieh, F.-J., and Tsai, H.-J. (2003) Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 228(1):30-40
Hsiao, C.-D., Tsai, W.-Y., Horng, L.-S., and Tsai, H.-J. (2003) Molecular structure and developmental expression of three muscle-type troponin T genes in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 227:266-279
Hsiao, C.D., Tsai, W.Y., and Tsai, H.J. (2002) Isolation and expression of two zebrafish homologues of parvalbumin genes related to chicken CPV3 and mammalian oncomodulin. Mechanisms of Development. (Suppl.) 119:S161-S166
Chen, Y.-H. and Tsai, H.-J. (2002) Treatment with Myf5-morpholino results in somite patterning and brain formation defects in zebrafish. Differentiation; research in biological diversity. 70(8):447-456
Ho, Y.-L., Shau, Y.-W., Tsai, H.-J., Lin, L.-C., Huang, P.-J., and Hsieh, F.-J. (2002) Assessment of zebrafish cardiac performance using Doppler echocardiography and power angiography. Ultrasound Med. Biol.. 28(9):1137-1143
Wang, T.-M., Chen, Y.-H., Liu, C.-F., and Tsai, H.-J. (2002) Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis. Mar. Biotechnol.. 4(3):247-255
Chen, Y.-H., Liang, C.-T., and Tsai, H.-J. (2002) Expression, purification and DNA-binding activity of tilapia muscle-specific transcription factor, MyoD, produced in Escherichia coli. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 131(4):795-805
Hsiao, C.D., Hsieh, F.J., and Tsai, H.J. (2001) Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno-associated virus in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 220(4):323-336
Chen, Y.-H., Lee, W.-C., Liu, C.-F., and Tsai, H.-J. (2001) Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene. Genesis (New York, N.Y. : 2000). 29(1):22-35
Chen, Y.-H., Lee, W.-C., Cheng, C.-H., and Tsai, H.-J. (2000) Muscle regulatory factor gene: zebrafish (Danio rerio) myogenin cDNA. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 127(1):97-103

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