PUBLICATION
A neuronal blueprint for directional mechanosensation in larval zebrafish
- Authors
- Valera, G., Markov, D.A., Bijari, K., Randlett, O., Asgharsharghi, A., Baudoin, J.P., Ascoli, G.A., Portugues, R., López-Schier, H.
- ID
- ZDB-PUB-210207-8
- Date
- 2021
- Source
- Current biology : CB 31(7): 1463-1475.e6 (Journal)
- Registered Authors
- Keywords
- hair cells, lateral line, mechanosensation, neurons, projectome, rheotaxis, single-cell tracing, somatotopy
- MeSH Terms
-
- Animals
- Larva
- Lateral Line System*
- Mechanoreceptors
- Orientation, Spatial*
- Zebrafish*
- PubMed
- 33545047 Full text @ Curr. Biol.
Citation
Valera, G., Markov, D.A., Bijari, K., Randlett, O., Asgharsharghi, A., Baudoin, J.P., Ascoli, G.A., Portugues, R., López-Schier, H. (2021) A neuronal blueprint for directional mechanosensation in larval zebrafish. Current biology : CB. 31(7):1463-1475.e6.
Abstract
Animals have a remarkable ability to use local cues to orient in space in the absence of a panoramic fixed reference frame. Here we use the mechanosensory lateral line in larval zebrafish to understand rheotaxis, an innate oriented swimming evoked by water currents. We generated a comprehensive light-microscopy cell-resolution projectome of lateralis afferent neurons (LANs) and used clustering techniques for morphological classification. We find surprising structural constancy among LANs. Laser-mediated microlesions indicate that precise topographic mapping of lateral-line receptors is not essential for rheotaxis. Recording neuronal-activity during controlled mechanical stimulation of neuromasts reveals unequal representation of water-flow direction in the hindbrain. We explored potential circuit architectures constrained by anatomical and functional data to suggest a parsimonious model under which the integration of lateralized signals transmitted by direction-selective LANs underlies the encoding of water-flow direction in the brain. These data provide a new framework to understand how animals use local mechanical cues to orient in space.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping