PUBLICATION
Kisspeptin-1 regulates forebrain dopaminergic neurons in the zebrafish
- Authors
- Abdul Satar, N.M., Ogawa, S., Parhar, I.S.
- ID
- ZDB-PUB-201120-79
- Date
- 2020
- Source
- Scientific Reports 10: 19361 (Journal)
- Registered Authors
- Ogawa, Satoshi
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal
- Chromatography, Liquid
- Dopamine/metabolism
- Dopaminergic Neurons/metabolism*
- Gene Expression Regulation
- Habenula/metabolism*
- Interneurons/metabolism
- Kisspeptins/metabolism*
- Male
- Neurons/metabolism
- Prosencephalon/metabolism*
- RNA, Messenger/metabolism
- Raphe Nuclei/metabolism
- Real-Time Polymerase Chain Reaction
- Social Behavior
- Tandem Mass Spectrometry
- Telencephalon/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 33168887 Full text @ Sci. Rep.
Citation
Abdul Satar, N.M., Ogawa, S., Parhar, I.S. (2020) Kisspeptin-1 regulates forebrain dopaminergic neurons in the zebrafish. Scientific Reports. 10:19361.
Abstract
The habenula is a phylogenetically conserved epithalamic structure, which conveys negative information via inhibition of mesolimbic dopamine neurons. We have previously shown the expression of kisspeptin (Kiss1) in the habenula and its role in the modulation of fear responses in the zebrafish. In this study, to investigate whether habenular Kiss1 regulates fear responses via dopamine neurons in the zebrafish, Kiss1 peptides were intracranially administered close to the habenula, and the expression of dopamine-related genes (th1, th2 and dat) were examined in the brain using real-time PCR and dopamine levels using LC-MS/MS. th1 mRNA levels and dopamine levels were significantly increased in the telencephalon 24-h and 30-min after Kiss1 administration, respectively. In fish administered with Kiss1, expression of neural activity marker gene, npas4a and kiss1 gene were significantly decreased in the ventral habenula. Application of neural tracer into the median raphe, site of habenular Kiss1 neural terminal projections showed tracer-labelled projections in the medial forebrain bundle towards the telencephalon where dopamine neurons reside. These results suggest that Kiss1 negatively regulates its own neuronal activity in the ventral habenula via autocrine action. This, in turn affects neurons of the median raphe via interneurons, which project to the telencephalic dopaminergic neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping