PUBLICATION
Hexavalent chromium amplifies the developmental toxicity of graphene oxide during zebrafish embryogenesis
- Authors
- Chen, Y., Li, J., Zhou, Q., Liu, Z., Li, Q.
- ID
- ZDB-PUB-201031-23
- Date
- 2020
- Source
- Ecotoxicology and environmental safety 208: 111487 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Heavy metals, Joint toxicity, Nanomaterials, Teratogenicity
- MeSH Terms
-
- Animals
- Chromium/toxicity*
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects*
- Graphite/toxicity*
- Nanoparticles/toxicity
- Toxicity Tests
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- PubMed
- 33126181 Full text @ Ecotoxicol. Environ. Saf.
Citation
Chen, Y., Li, J., Zhou, Q., Liu, Z., Li, Q. (2020) Hexavalent chromium amplifies the developmental toxicity of graphene oxide during zebrafish embryogenesis. Ecotoxicology and environmental safety. 208:111487.
Abstract
Combined toxicity is a critical issue in risk assessment of contaminants. However, very little is known about the joint effects of graphene oxide (GO, a crucial 2-dimensional carbon material) and hexavalent chromium (Cr6+, a widespread heavy metal), particularly with respect to the critical period of embryogenesis. In this study, the combined toxicity of GO and Cr6+ was evaluated through embryo-larval toxicity test in Danio rerio (zebrafish). Results indicated that the co-exposure of Cr6+ (1 mg/L) and GO (0.01 mg/L) inhibited hatching and spontaneous movement of embryos, but no significant changes were found in the single Cr6+ or GO group. Compared with the single GO or Cr6+ exposure, their co-exposure (GO+Cr6+) significantly enhanced the teratogenicity in a concentration-dependent pattern, and the spinal curvature was observed as the main deformity. GO+Cr6+ changed the protein secondary structures of embryos result of the generation of ROS and oxidative stress. The degradations of vertical myosepta and cartilages were observed in co-exposure group, suggesting that GO+Cr6+ disrupted the development of musculoskeletal system. The genes col11a1a, col2a1a and postnb were down-regulated but the genes acta1b and mmp9 were up-regulated by GO+Cr6+. The interactions between Cr6+ and GO demonstrated that the morphology, structure, and surface properties of GO were modified by Cr6+. The enhanced defects and O-containing groups of GO could trap more β-sheets, induced oxidative stress, disturbed the development of skeletal muscles and cartilages in zebrafish. These data suggested that GO+Cr6+ enhanced their joint toxicity due to the variation of nanoparticle properties. This finding is important for assessing the ecological risk of graphene family nanomaterials in the natural environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping