PUBLICATION
Inducible modulation of miR-204 levels in a zebrafish melanoma model
- Authors
- Sarti, S., De Paolo, R., Ippolito, C., Pucci, A., Pitto, L., Poliseno, L.
- ID
- ZDB-PUB-201011-8
- Date
- 2020
- Source
- Biology Open 9(11): (Journal)
- Registered Authors
- Keywords
- Melanoma model, MiR-204, MiniCoopR-I, Pigmentation, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Disease Models, Animal
- Gene Expression
- Gene Expression Regulation, Neoplastic*
- Gene Knockout Techniques
- Gene Order
- Genetic Vectors/genetics
- Humans
- Immunohistochemistry
- Melanocytes/metabolism
- Melanoma/genetics*
- Melanoma/pathology
- MicroRNAs/genetics*
- Zebrafish
- PubMed
- 33037013 Full text @ Biol. Open
Citation
Sarti, S., De Paolo, R., Ippolito, C., Pucci, A., Pitto, L., Poliseno, L. (2020) Inducible modulation of miR-204 levels in a zebrafish melanoma model. Biology Open. 9(11):.
Abstract
Here, we present miniCoopR-I, an inducible upgrade of the constitutive miniCoopR vector. We developed miniCoopR-I-sponge-204 and miniCoopR-I-pre-miR-204 vectors and we successfully tested them for their ability to achieve time (embryo/juvenile/adult)- and space (melanocytic lineage)- restricted inhibition/overexpression of miR-204, a positive modulator of pigmentation previously discovered by us. Furthermore, melanoma-free survival curves performed on induced fish at adult stage indicate that miR-204 overexpression accelerates the development of BRAFV600E-driven melanoma. miniCoopR-I allows to study the impact that coding and non-coding modulators of pigmentation exert on melanomagenesis in adult zebrafish, uncoupling it from the impact that they exert on melanogenesis during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping